IDENTIFICATION OF COMMON PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND
SURFACE OF RAT SPERMATOZOA

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SADEGHI M.R.; AKHOUNDI M.A.; ZAHRAEI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: Research in Medicine Year:2001 | Volume:25 | Issue:1 Page(s): 43-54

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Information Journal Paper

Title

IDENTIFICATION OF COMMON PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA

Author(s)

SADEGHI M.R. | AKHOUNDI M.A. | ZAHRAEI M. | Issue Writer Certificate

Keywords

EPIDIDYMISQ3

SPERMQ2

RATQ2

SPERM MATURATIONQ4

SECRETORY PROTEINSQ4

SECRETORY GLYCOPROTEINSQ4

Abstract

After the SPERM leaving the testis, further post testicular development is required so that fully functional SPERMatozoa capable of passing through the female tract and fertilizing ova, produce. This process of SPERM maturation occurs in EPIDIDYMIS. The epididymal epithelium provides the special microenvironment for SPERM - maturation. This process involved remodeling, insertion, deletion or modification of many proteins and lipid components of SPERM plasma membrane. Evaluation of such modification has a critical role in understanding of epididymal function. Segments of washed caput and corpus regions of RAT EPIDIDYMIS were treated with enzymatic and mechanical digestion to remove connective tissue and produce fragments less than 1mm length. Epididymal fragments were successfully cultured in a modified RPMI-1640 medium supplemented with androgens and FCS. Pulse labeling of epididymal culture wereperformedin vitrowith [35S]Metand [35S]Cys mixture.SDS-PAGEand flurography evaluated protein synthesis and secretion. An antiserum against SPERM surface proteins was raised by repeated injections of RAT viable SPERMatozoa to a rabbit. ELISA and indirect immunoflurescencetechniques checked the antiserum. Identical proteins in epididymal secretions and SPERM surface were identified by western blotting. Epididymal epithelia in culture displayed about 20 distinct protein bands. The most intense bands revealed to be at approximately 20-200 KD. A number of bands were evident less intensity. Immune serum reacted with 20, 24. 54,58 KD protein bands after western blotting and were common between SPERM surface and epididymal epithelia secretions. Epithelial cells of EPIDIDYMIS secrete proteins and glycoproteins, some of them binded to SPERM surface and probably have critical roles in SPERM-oocyte interaction or membrane fusion. We have identified 4 proteins with molecular mass of 20, 24, 54, 58 KD that secreted by epididymal epithelia and bounded to SPERM surface. Our future research will focus on purification ofthe proteins and evaluation of their roles on fertilization process and SPERM fertilizing ability by IVF techniques.

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APA: Copy

SADEGHI, M.R., AKHOUNDI, M.A., & ZAHRAEI, M.. (2001). IDENTIFICATION OF COMMON PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA. PAJOUHESH DAR PEZESHKI, 25(1), 43-54. SID. https://sid.ir/paper/42005/en

Vancouver: Copy

SADEGHI M.R., AKHOUNDI M.A., ZAHRAEI M.. IDENTIFICATION OF COMMON PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA. PAJOUHESH DAR PEZESHKI[Internet]. 2001;25(1):43-54. Available from: https://sid.ir/paper/42005/en

IEEE: Copy

M.R. SADEGHI, M.A. AKHOUNDI, and M. ZAHRAEI, “IDENTIFICATION OF COMMON PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA,” PAJOUHESH DAR PEZESHKI, vol. 25, no. 1, pp. 43–54, 2001, [Online]. Available: https://sid.ir/paper/42005/en

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