COMPARISON OF THREE DIAGNOSTIC TESTS: HISTOLOGY, SEROLOGY AND RAPID UREASE TEST (RUT) IN IDENTIFICATION OF HELICOBACTER PYLORI INFECTION IN CHILDREN

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ESMAEELI M.R.; MORADI SIAVASH

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Journal: Journal of Mazandaran University of Medical Sciences Year:2002 | Volume:12 | Issue:36 Page(s): 15-23

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Title

COMPARISON OF THREE DIAGNOSTIC TESTS: HISTOLOGY, SEROLOGY AND RAPID UREASE TEST (RUT) IN IDENTIFICATION OF HELICOBACTER PYLORI INFECTION IN CHILDREN

Author(s)

ESMAEELI M.R. | MORADI SIAVASH | Issue Writer Certificate

Keywords

HELICOBACTER PYLORIQ3

CHILDRENQ3

SERODIAGNOSISQ4

IMMUNOLOGICAL TESTSQ4

DIAGNOSISQ3

Abstract

Background and purpose: HELICOBACTER PYLORI (H.pylori) is responsible for causing many diseases in CHILDREN. In the recent years different methods have been used to identify this infection. The aim of this study is to compare the results of above mentioned methods in identification of H.pylori in CHILDREN.Materials and methods : This prospective study was done on 50 infants aged 3 to 14 years having various complaints under went upper gastrointestinal endoscopy in Amir kolah pediatrics hospital in the year 2000. Biopsy specimen was obtained for microbiological examination (Giemsa staining) and urease test in BIRD medium. Blood was collected from the patients for serologic test (using RADIM kit). The standard ratio for identification of H.pylori infection in this study was indicated as at least two positive tests out of three tests.Results: Out of 50 infants under investigation, 19 (38%) were infected with H.pylori. Frequency distribution of this infection in boys was 45% and in girls 96.3% (P>0.05). In this study the presence of IgG against H.pylori was determined by ELISA with cut off=23 ur/ml. The sensitivity and specificity for ELISA, histology and urease test was as follow: 89.4% and 96.7%, 89.4% and 96.7, 89.4% and 100% respectively.Conclusion: It is concluded from this study that, through standardization of ELISA, the above mentioned three methods of the tests have the same sensitivity property. Though rapid urease test with higher specificity is more reliable method, but without standardization of ELISA in infants the sensitivity and specificity would be lower.

Cites

PREVALENCE OF HELICOBACTER PYLORI INFECTION IN TYPE 1 DIABETIC CHILDREN REFERRING TO ISFAHAN ENDOCRINE & METABOLISM RESEARCH CENTER

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APA: Copy

ESMAEELI, M.R., & MORADI, SIAVASH. (2002). COMPARISON OF THREE DIAGNOSTIC TESTS: HISTOLOGY, SEROLOGY AND RAPID UREASE TEST (RUT) IN IDENTIFICATION OF HELICOBACTER PYLORI INFECTION IN CHILDREN. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 12(36), 15-23. SID. https://sid.ir/paper/45063/en

Vancouver: Copy

ESMAEELI M.R., MORADI SIAVASH. COMPARISON OF THREE DIAGNOSTIC TESTS: HISTOLOGY, SEROLOGY AND RAPID UREASE TEST (RUT) IN IDENTIFICATION OF HELICOBACTER PYLORI INFECTION IN CHILDREN. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;12(36):15-23. Available from: https://sid.ir/paper/45063/en

IEEE: Copy

M.R. ESMAEELI, and SIAVASH MORADI, “COMPARISON OF THREE DIAGNOSTIC TESTS: HISTOLOGY, SEROLOGY AND RAPID UREASE TEST (RUT) IN IDENTIFICATION OF HELICOBACTER PYLORI INFECTION IN CHILDREN,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 12, no. 36, pp. 15–23, 2002, [Online]. Available: https://sid.ir/paper/45063/en

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