IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES BY PCR-RFLP TECHNIQUE IN ISFAHAN

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BAGHAEI M.; PESTEHCHIAN N.; SALEHI MANSOUR; KAZEMI B.; MIRMOHAMMAD SADEGHI HAMID

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Journal: Journal of Isfahan Medical School Year:2005 | Volume:23 | Issue:77 Page(s): 42-48

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Title

IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES BY PCR-RFLP TECHNIQUE IN ISFAHAN

Author(s)

Keywords

TRICHOSTRONGYLUSQ4

PCR-RFLP TECHNIQUEQ4

RDNA-ITS2Q4

RSAI-HINFI-DRAIQ4

Abstract

Introduction: Trichostrongyliasis is a zoonotic disease of major public health and socioeconomic importance. Accurate identification of the parasite is the first step in strategic planning for the prevention, control, and treatment of infestation, and for better understanding of the epidemiology of this parasitic disease.Materials and methods: Samples were collected from alimentary tract content of 56 sheep and 53goats in Isfahan and Khorasgan slaughterhouses; identification of TRICHOSTRONGYLUS was conducted based on morphological character and key identification and stained copulatory bursa with azocarmine dye. For genotyping studies, DNA was extracted and the ITS-2 of ribosomal DNA of each species with specific primers was amplified by PCR and restricted with the endonucleases RsaI-Hinfl-DraI. The profiles were visualized in agarose gel under ultraviolet transillumination.Results: Based on the morphological character, four TRICHOSTRONGYLUS species, namely T. axei, T. vitrinus, T. colubriformis, T. probulurus were identified. RDNA-ITS2 fragment size of all species were the same size (about 330bp), however, there were differences between species in their PCR-RFLP patterns. Two fragments were produced with RsaI in all species and were the same size (about 138bp, 190bp). The PCR product T. probulurus with DraI remained .unrestricted. Restriction with DraI produced two fragments in the PCR product of T. axei and T. colubriformis (about 110bp and 215bp, respectively); however, the larger of the two fragments in T. vitrinus is smaller in size than in T. axei and T. colubriformis (about l45bp and 185bp, respectively). Two fragments were produced with Hinfl in T. colubriformis (about 90bp and 238bp, respectively). By contrast, other TRICHOSTRONGYLUS remained unrestricted.Discussion: Based on our results from both morphological and genotyping studies using PCR-RFLP TECHNIQUE, it can be concluded that four species were identified. This study provides a pattern for differentiation of TRICHOSTRONGYLUS.species in Isfahan region using the RsaI-Hinfl-DraI restriction endonucleases.

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STUDY ON FOUR TRICHOSTRONGYLUS SPECIES IN ISFAHAN BY PCR-RFLP

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APA: Copy

BAGHAEI, M., PESTEHCHIAN, N., SALEHI, MANSOUR, KAZEMI, B., & MIRMOHAMMAD SADEGHI, HAMID. (2005). IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES BY PCR-RFLP TECHNIQUE IN ISFAHAN. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 23(77), 42-48. SID. https://sid.ir/paper/50322/en

Vancouver: Copy

BAGHAEI M., PESTEHCHIAN N., SALEHI MANSOUR, KAZEMI B., MIRMOHAMMAD SADEGHI HAMID. IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES BY PCR-RFLP TECHNIQUE IN ISFAHAN. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2005;23(77):42-48. Available from: https://sid.ir/paper/50322/en

IEEE: Copy

M. BAGHAEI, N. PESTEHCHIAN, MANSOUR SALEHI, B. KAZEMI, and HAMID MIRMOHAMMAD SADEGHI, “IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES BY PCR-RFLP TECHNIQUE IN ISFAHAN,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 23, no. 77, pp. 42–48, 2005, [Online]. Available: https://sid.ir/paper/50322/en

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