STUDY ON FOUR TRICHOSTRONGYLUS SPECIES IN ISFAHAN BY PCR-RFLP

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PESTEHCHIAN N.; BAGHAEI M.; YOUSEFI H.A.

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Journal Paper

Paper Information

Journal: Feyz Medical Sciences Journal Year:2005 | Volume:9 | Issue:3 (SERIAL 35) Page(s): 23-29

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Title

STUDY ON FOUR TRICHOSTRONGYLUS SPECIES IN ISFAHAN BY PCR-RFLP

Author(s)

PESTEHCHIAN N. | BAGHAEI M. | YOUSEFI H.A. | Issue Writer Certificate

Keywords

TRICHOSTRONGYLUSQ3

TAXONOMYQ2

IRANQ4

PCR-RFLPQ2

RDNA ITS2Q2

RSALQ4

HINFIQ3

DRALQ4

Abstract

Background: Trichostrongyliases as zoonotic diseases are of major public health and sodo-economic importance. Accurate identification of these parasites is the first step in strategic planning for the prevention, control and treatment of the parasitic disease. The aim of this study is using PCR-RFLP technique for genotyping.Materials and methods: samples were collected from elementary tract content of 70 sheep in lsfahan and Khorasgan slaughterhouses. Identification of TRICHOSTRONGYLUS was determined based on morphological character and key identification after direct and flotation methods. For genotyping studies, DNA was extracted and the ITS-2 of ribosomal DNA of each species with specific primers was amplified by PCR and restricted with the endonucleases DraI, RsaI, HINFI. The profile was visualized in agarose gel under ultraviolet transillumination.Results: The eggs of most species are morphologically indistinguishable at the generic level .rDNA-ITS2 fragment size of all species were the same size about 330bp, however, there were differences among species in their PCR-RFLP patterns. The two fragments produced with RSAL in all species were the same size about 138bp, 190bp. The PCR product Tprobulurus with DRAL remained unrestricted. Restriction with DRAL produced two fragments in the PCR product of Taxei and Tcolubriformis about 110bp, 215bp, however, the larger of the two fragments in T.vitrinus is smaller in size than in Taxei and T.colubrtformis had a size about 145bp, 185bp. The two fragments produced with HINFI in T.colubriformis had a size about 90bp, 238bp, By contrast other TRICHOSTRONGYLUS remained unrestricted.Conclusion: Based of our results from genotyping studies using PCR-RFLP technique, it can be concluded that four species had been identified. This study provides a pattern to distinguish TRICHOSTRONGYLUS species in Isfahan region using the DraI, RsaI HINFI restriction endonucleases.

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References

IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES BY PCR-RFLP TECHNIQUE IN ISFAHAN

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APA: Copy

PESTEHCHIAN, N., BAGHAEI, M., & YOUSEFI, H.A.. (2005). STUDY ON FOUR TRICHOSTRONGYLUS SPECIES IN ISFAHAN BY PCR-RFLP. FEYZ, 9(3 (SERIAL 35)), 23-29. SID. https://sid.ir/paper/40940/en

Vancouver: Copy

PESTEHCHIAN N., BAGHAEI M., YOUSEFI H.A.. STUDY ON FOUR TRICHOSTRONGYLUS SPECIES IN ISFAHAN BY PCR-RFLP. FEYZ[Internet]. 2005;9(3 (SERIAL 35)):23-29. Available from: https://sid.ir/paper/40940/en

IEEE: Copy

N. PESTEHCHIAN, M. BAGHAEI, and H.A. YOUSEFI, “STUDY ON FOUR TRICHOSTRONGYLUS SPECIES IN ISFAHAN BY PCR-RFLP,” FEYZ, vol. 9, no. 3 (SERIAL 35), pp. 23–29, 2005, [Online]. Available: https://sid.ir/paper/40940/en

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