GIARDIACIDAL ACTIVITY OF THE EXPRESS OBTAINED FROM BULBS OF THREE ALLIUM SPECIES ON GIARDIA INTESTINALIS CYSTS

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AZADBAKHT M.; SAJADI S.M.; ROSTAMI J.

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Title

GIARDIACIDAL ACTIVITY OF THE EXPRESS OBTAINED FROM BULBS OF THREE ALLIUM SPECIES ON GIARDIA INTESTINALIS CYSTS

Author(s)

AZADBAKHT M. | SAJADI S.M. | ROSTAMI J. | Issue Writer Certificate

Keywords

GIARDIAQ3

ALLIUMQ3

A. SATIVUMQ3

A. CEPAQ2

A. ASCALONICUMQ3

Abstract

A study was performed in order to investigate the efficacy of simple disinfecting procedure for three ALLIUM species (A. sativum, A cepa, A. ASCALONICUM) on GIARDIA cysts. The cysts were obtained from stools of individuals with GIARDIA intestinalis infection using a modified sucrose gradient procedure. A pool batch of GIARDIA cyst containing 3× 104/ ml GIARDIA cysts was prepared from all specimens. The cysts were kept at 4°c until use. Before each experiment, the numbers of cysts were determined by a hemocytometer. Two Sets of eppendorf tubes were used for the experiments. One set in 4°C and another in 24°C in each tube 100µl of each extract of the plants were poured and 100 µl of GIARDIA cysts for pooled specimen eppendorf tubes. Variables were disinfectant materials, temperature and time of exposure. The viability of cysts was determined using eosin inclusion procedure. The viability of at least 250 cysts in each tube at the times of 0, 0.5, 1, 2 and 3 hours after the beginning of experiments were determined. The mean GIARDIAcidal activity at 4°C for the A. SATIVUM, A. ascalonium and A. CEPA after 3 hours was 36.00%, 28.46%, 33.26%, and at 24°C was 43%, 33.86%, 41.13% respectively. In conclusion the GIARDIAcidal activity of A. SATIVUM is more than other species. The highest GIARDIAcidal activity of A. SATIVUM was 24°C temperature and after three hours.

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APA: Copy

AZADBAKHT, M., SAJADI, S.M., & ROSTAMI, J.. (2003). GIARDIACIDAL ACTIVITY OF THE EXPRESS OBTAINED FROM BULBS OF THREE ALLIUM SPECIES ON GIARDIA INTESTINALIS CYSTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 6(3 (19)), 184-188. SID. https://sid.ir/paper/52382/en

Vancouver: Copy

AZADBAKHT M., SAJADI S.M., ROSTAMI J.. GIARDIACIDAL ACTIVITY OF THE EXPRESS OBTAINED FROM BULBS OF THREE ALLIUM SPECIES ON GIARDIA INTESTINALIS CYSTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2003;6(3 (19)):184-188. Available from: https://sid.ir/paper/52382/en

IEEE: Copy

M. AZADBAKHT, S.M. SAJADI, and J. ROSTAMI, “GIARDIACIDAL ACTIVITY OF THE EXPRESS OBTAINED FROM BULBS OF THREE ALLIUM SPECIES ON GIARDIA INTESTINALIS CYSTS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 6, no. 3 (19), pp. 184–188, 2003, [Online]. Available: https://sid.ir/paper/52382/en

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