COMPARISON BETWEEN THE EFFECTS OF ISCHEMIC PRECONDITIONING AND PHARMACOLOGIC PRECONDITIONING BY L-CARNITINE ON INFARCT ZONE SIZE IN THE ISCHEMIC-REPERFUSED ISOLATED RAT HEART

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NAJAFI M.; GARJANI A.; ETERAF OSKOUEI T.

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Journal: Iranian Journal of Basic Medical Sciences Year:2007 | Volume:10 | Issue:1 (33) Page(s): 54-59

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Title

COMPARISON BETWEEN THE EFFECTS OF ISCHEMIC PRECONDITIONING AND PHARMACOLOGIC PRECONDITIONING BY L-CARNITINE ON INFARCT ZONE SIZE IN THE ISCHEMIC-REPERFUSED ISOLATED RAT HEART

Author(s)

NAJAFI M. | GARJANI A. | ETERAF OSKOUEI T. | Issue Writer Certificate

Keywords

PRECONDITIONINGQ3

L-CAMITINEQ2

ISCHEMIA-REPERFUSIONQ2

INFARCT SIZEQ3

ISOLATED HEARTQ3

Abstract

ObjectiveIschemic PRECONDITIONING (IP) is a process triggered by brief ischemia, which enables hearts to resist against injuries of prolonged period of ischemia. Carnitine is a vital biologic substance for transporting fatty acids into myocytes in order to produce energy. In this study, effects of IP and pharmacologic PRECONDITIONING (PP) by using L-Carnitine (L-Car) on INFARCT SIZE in the ischemic-reperfused isolated rat hearts were investigated and compared.Materials and MethodsMale Sprague-Dawley rats (270-330 g) were divided into five groups randomly (control, IP group, and three PP groups treated by L-Car, n=6 in each group) and were anesthetized by sodium pentobarbital (50 mg/kg-i.p). Heart was removed and quickly mounted on a Langendorff apparatus and perfused under constant pressure by a modified Krebs-Henseleit (K/H) solution that was previously equilibrated with 95% O2-5% CO2. The hearts were subjected to 30 min regional ischemia followed by 120 min reperfusion. In the control and IP groups, the hearts were perfused by normal K/H solution during stabilization, regional ischemia and reperfusion, while in the PP groups, they were perfused by 0.5, 2.5 and 5 mM of L-Car-enriched K/H solution 10 minbefore and 10 min after ischemia. At the end of reperfusion, Evans Blue solution was infused to stain the non-ischemic area. Then the heart was cut into slices, incubated by 1% triphenyltetrazolium chloride solution, and fixed by formalin. The area of infracted tissue and area at risk was determined by using a computerized planimetry package.ResultsInfarct size significantly was decreased in both IP and PP groups. In the control group, INFARCT SIZE was 46.3±2.9%, however, IP reduced it to 22.2±2.7% (p<0.001). Application of 0.5, 2.5 and 5mM of L – Car enriched K/H solution 10min before and after ischemia in the PP groups, markedly reduced INFARCT SIZE in the control group value to 25.9±3.0, 19.3±1.7 and 17.2±1.5%, respectively (p<0.001 for all). There was no significant difference between IP and PP groups on INFARCT SIZE reduction.Conclusion Considering the results, it may be concluded that IP and PP (by L-Car) have protective effects against cardiac ISCHEMIA-REPERFUSION injuries as a reduction of INFARCT SIZE without significant statistical difference between their effects.

Cites

EFFECTS OF CHRONIC ORAL ADMINISTRATION OF NATURAL HONEY ON ISCHEMIA/REPERFUSION-INDUCED ARRHYTHMIAS IN ISOLATED RAT HEART

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APA: Copy

NAJAFI, M., GARJANI, A., & ETERAF OSKOUEI, T.. (2007). COMPARISON BETWEEN THE EFFECTS OF ISCHEMIC PRECONDITIONING AND PHARMACOLOGIC PRECONDITIONING BY L-CARNITINE ON INFARCT ZONE SIZE IN THE ISCHEMIC-REPERFUSED ISOLATED RAT HEART. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 10(1 (33)), 54-59. SID. https://sid.ir/paper/52422/en

Vancouver: Copy

NAJAFI M., GARJANI A., ETERAF OSKOUEI T.. COMPARISON BETWEEN THE EFFECTS OF ISCHEMIC PRECONDITIONING AND PHARMACOLOGIC PRECONDITIONING BY L-CARNITINE ON INFARCT ZONE SIZE IN THE ISCHEMIC-REPERFUSED ISOLATED RAT HEART. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2007;10(1 (33)):54-59. Available from: https://sid.ir/paper/52422/en

IEEE: Copy

M. NAJAFI, A. GARJANI, and T. ETERAF OSKOUEI, “COMPARISON BETWEEN THE EFFECTS OF ISCHEMIC PRECONDITIONING AND PHARMACOLOGIC PRECONDITIONING BY L-CARNITINE ON INFARCT ZONE SIZE IN THE ISCHEMIC-REPERFUSED ISOLATED RAT HEART,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 10, no. 1 (33), pp. 54–59, 2007, [Online]. Available: https://sid.ir/paper/52422/en

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