EFFECTS OF ETHANOLIC EXTRACT OF ALPINIA GALANGAL L ON PROLIFERATION OF HT-29 AND L929 CELL LINES IN VITRO

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HAJZADEH M.A.R.; TAVAKOL AFSHARI J.; BARATI MINA

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Journal: Studies in Medical Sciences Year:2007 | Volume:18 | Issue:3 Page(s): 572-581

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Title

EFFECTS OF ETHANOLIC EXTRACT OF ALPINIA GALANGAL L ON PROLIFERATION OF HT-29 AND L929 CELL LINES IN VITRO

Author(s)

HAJZADEH M.A.R. | TAVAKOL AFSHARI J. | BARATI MINA | Issue Writer Certificate

Keywords

PHYSIOLOGY DEPARTMENTQ3

GHAEM HOSPITALQ3

MASHHAD UNIVERSITY OF MEDICAL SCIENCES. MASHHADQ2

IRANQ3

Abstract

Background & Aims: Colon adenocarcinoma is a prevalent cancer throughout the world. The incidence of this cancer is 3.39% of all malignant tumors in Khorasan province (North-East of IRAN).Although the surgical removal of the adenocarcinoma is the main route of treatment at present, the survival is not so good in most patients. It seems that any other approach for the treatment of this cancer with possible eradication of tumor or improvement of survival rate and duration is of great interest.Alpinia galanga L is a medicinal plant. Various therapeutic effects such as antibacterial, analgesic and anti- inflammatory, vasodilatory, neuronal protective and hypoglycemic effects have been reported about this plat. Several studies have also demonstrated anticancer effects, growth inhibitory, and cytotoxic effects for Alpinia galanga or its different components. The anticancer effects of these components are due to scavenging of free radicals, inhibition of enzyme activities and inhibition of cytotoxicity. The aim of this study was to investigate the possible effects of ethanolic extract of Alpinia galanga on proliferation and growth of HT-29 (human adenocarcinoma of colon) as case and L929 (normal mouse fibroblast) cell lines as control.Materials & Methods: Ethanolic extract of rhizemes from Alpinia galanga were prepared by soxhelet and both cell lines were studied by morphological and MTT assays. Both HT-29 and L929 cell lines were treated with equivalent of 250, 500, 750, 1000 and 1500 mg/ml of Alpinia galanga from the ethanolic extract in culture medium for intervals of 24,48 and 72 hours. Both cell lines were studied under light inverted microscopes for shape, granulation, and anchorage independency. At the same time anti-proliferative properties of the extract were determined for both cell lines by MTT colorimetric assay. The results were compared by one-way ANOVA followed by Tukey-Kramer and when P-values were less than 0.05, the differences were considered to be significant.Results: The data demonstrated that ethanolic extract of the plant had inhibitory effects on HT-29 cell line but no inhibitory effects were seen for L929 cell line. The IC50 for HT-29 cell line with ethanolic extract at 72 hour was 250 mg/ml.Conclusion: In conclusion, these data demonstrate that ethanolic extract is effective and exert inhibitory effects on growth of HT-29 cell line. Therefore, it may be suggested that the ethanolic extract of Alpinia galanga L. would have inhibitory and protective effects on human colon adenocarcinoma and may be used in treatment of adenocarcinoma of colone in man. Keywords: Alpinia galanga L, Ethanolic extract, Colon adenocarcinoma, HT- 29 cells, L929 cells.

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APA: Copy

HAJZADEH, M.A.R., TAVAKOL AFSHARI, J., & BARATI, MINA. (2007). EFFECTS OF ETHANOLIC EXTRACT OF ALPINIA GALANGAL L ON PROLIFERATION OF HT-29 AND L929 CELL LINES IN VITRO. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 18(3), 572-581. SID. https://sid.ir/paper/64122/en

Vancouver: Copy

HAJZADEH M.A.R., TAVAKOL AFSHARI J., BARATI MINA. EFFECTS OF ETHANOLIC EXTRACT OF ALPINIA GALANGAL L ON PROLIFERATION OF HT-29 AND L929 CELL LINES IN VITRO. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2007;18(3):572-581. Available from: https://sid.ir/paper/64122/en

IEEE: Copy

M.A.R. HAJZADEH, J. TAVAKOL AFSHARI, and MINA BARATI, “EFFECTS OF ETHANOLIC EXTRACT OF ALPINIA GALANGAL L ON PROLIFERATION OF HT-29 AND L929 CELL LINES IN VITRO,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 18, no. 3, pp. 572–581, 2007, [Online]. Available: https://sid.ir/paper/64122/en

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