Effect of the Internal Septum Extract of theWalnut Kernel on the Mesenchymal Stem Cells Cycle and MSCs-derived Insulin-Producing B-cells Differentiation and Glucose Uptake

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Aghapour SK. Fatemeh; SISAKHTNEZHAD SAJJAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jentashapir Journal of Cellular and Molecular Biology Year:2021 | Volume:12 | Issue:2 Page(s): 0-0

Download Full-Text

View:

Download:

128

Cites:

Information Journal Paper

Title

Effect of the Internal Septum Extract of theWalnut Kernel on the Mesenchymal Stem Cells Cycle and MSCs-derived Insulin-Producing B-cells Differentiation and Glucose Uptake

Author(s)

Aghapour SK. Fatemeh | SISAKHTNEZHAD SAJJAD | Issue Writer Certificate

Keywords

Mesenchymal Stem Cell

Insulin-ProducingB-cell

Proliferation

Differentiation

Glucose Uptake

Walnut Septum

Abstract

Background: For years, scientists have been striving to identify compounds to improve the characteristics and functions of cells for therapeutic applications. Objectives: This study aimed to evaluate the effect of the aqueous decoction of the internal septum of the walnut kernel (DISWK) on the survival, total antioxidant capacity (TAC), and cell cycle of rat bone marrow-derivedMesenchymal Stem Cells (rBM-MSCs). We also evaluated the expression of genes involved in pancreatic insulin-producing -cell (IPCs) commitment and Glucose Uptake in MSCs-derived IPCs. Methods: The rBM-MSCs were isolated, and then the cytotoxic and antioxidant effects of DISWK on the cells were examined by the MTT and TAC assays, respectively. Moreover, the influence of DISWK on the rBM-MSCs cell cycle was evaluated by PI-staining and flowcytometry analysis. Next, the rBM-MSCs were transdifferentiated into IPCs treated with DISWK. Finally, Ins1/2, Insr, Glut1, Glut4, and Pdx1 genes expression was investigated in the DISWK-treated IPCs by qRT-PCR. Results: The results indicated thatDISWKsignificantly reduced the viability of rBM-MSCs in a dose-andtime-dependentmannerand decreased their TAC in long-term cultures. Moreover, it suppressed the rBM-MSCs cell cycle at S and G2 phases. The gene expression analysis showed that DISWK significantly upregulated Ins1/2, Insr, and Glut1 genes and downregulated Pdx1 gene in the rBM-MSCsderived IPCs. Conclusions: Decoction of the internal septum of walnut kernel suppresses the MSCs cell cycle and may prevent IPCs development via inhibiting Pdx1. It may also help Glucose Uptake by upregulating Ins1/2, Insr, and Glut1 genes expression in MSCs-derived IPCs.

Cites

No record.

References

Cite

APA: Copy

Aghapour SK., Fatemeh, & SISAKHTNEZHAD, SAJJAD. (2021). Effect of the Internal Septum Extract of theWalnut Kernel on the Mesenchymal Stem Cells Cycle and MSCs-derived Insulin-Producing B-cells Differentiation and Glucose Uptake. JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH), 12(2), 0-0. SID. https://sid.ir/paper/701090/en

Vancouver: Copy

Aghapour SK. Fatemeh, SISAKHTNEZHAD SAJJAD. Effect of the Internal Septum Extract of theWalnut Kernel on the Mesenchymal Stem Cells Cycle and MSCs-derived Insulin-Producing B-cells Differentiation and Glucose Uptake. JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH)[Internet]. 2021;12(2):0-0. Available from: https://sid.ir/paper/701090/en

IEEE: Copy

Fatemeh Aghapour SK., and SAJJAD SISAKHTNEZHAD, “Effect of the Internal Septum Extract of theWalnut Kernel on the Mesenchymal Stem Cells Cycle and MSCs-derived Insulin-Producing B-cells Differentiation and Glucose Uptake,” JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH), vol. 12, no. 2, pp. 0–0, 2021, [Online]. Available: https://sid.ir/paper/701090/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops