Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers

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Borzouie Zahra; Naghibzadeh Majid; TALEBI ALI REZA; POURRAJAB FATEMEH; JEBALI ALI; Nikukar Habib; Molla Hoseini Hosein; KHORADMEHR AREZOO; Sadeghian Nodoushan Fatemeh; AFLATOONIAN BEHROUZ; HEKMATI MOGHADDAM SEYEDHOSSEIN

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Journal Paper

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Journal: Cell Journal (Yakhteh) Year:2019 | Volume:21 | Issue:3 Page(s): 300-306

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Title

Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers

Author(s)

Keywords

Azoospermia

Human Serum Albumin

Scaffold

Testis

Tissue Engineering

Abstract

Objective: Recent achievements in stem cell biotechnology, nanotechnology and Tissue Engineering have led to development of novel approaches in regenerative medicine. Azoospermia is one of the challenging disorders of the reproductive system. Several efforts were made for isolation and culture of Testis-derived stem cells to treat male infertility. However, Tissue Engineering is the best approach to mimic the three dimensional microenvironment of the Testis in vitro. We investigated whether human Testis-derived cells (hTCs) obtained by testicular sperm extraction (TESE) can be cultured on a homemade Scaffold composed of electrospun nanofibers of homogeneous poly (vinyl alcohol)/Human Serum Albumin/gelatin (PVA/HSA/gelatin). Materials and Methods: In this experimental lab study, human TCs underwent two steps of enzymatic cell isolation and five culture passages. Nanofibrous Scaffolds were characterized by scanning electron microscopy (SEM) and Fouriertransform infrared spectroscopy (FTIR). Attachment of cells onto the Scaffold was shown by hematoxylin and eosin (H&E) staining and SEM. Cell viability study using MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] assay was performed on days 7 and 14. Results: Visualization by H&E staining and SEM indicated that hTCs were seeded on the Scaffold. MTT test showed that the PVA/HSA/gelatin Scaffold is not toxic for hTCs. Conclusion: It seems that this PVA/HSA/gelatin Scaffold is supportive for growth of hTCs.

Cites

The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche

References

Efficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells

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APA: Copy

Borzouie, Zahra, Naghibzadeh, Majid, TALEBI, ALI REZA, POURRAJAB, FATEMEH, JEBALI, ALI, Nikukar, Habib, Molla Hoseini, Hosein, KHORADMEHR, AREZOO, Sadeghian Nodoushan, Fatemeh, & HEKMATI MOGHADDAM, SEYEDHOSSEIN. (2019). Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers. CELL JOURNAL (YAKHTEH), 21(3), 300-306. SID. https://sid.ir/paper/718504/en

Vancouver: Copy

Borzouie Zahra, Naghibzadeh Majid, TALEBI ALI REZA, POURRAJAB FATEMEH, JEBALI ALI, Nikukar Habib, Molla Hoseini Hosein, KHORADMEHR AREZOO, Sadeghian Nodoushan Fatemeh, HEKMATI MOGHADDAM SEYEDHOSSEIN. Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers. CELL JOURNAL (YAKHTEH)[Internet]. 2019;21(3):300-306. Available from: https://sid.ir/paper/718504/en

IEEE: Copy

Zahra Borzouie, Majid Naghibzadeh, ALI REZA TALEBI, FATEMEH POURRAJAB, ALI JEBALI, Habib Nikukar, Hosein Molla Hoseini, AREZOO KHORADMEHR, Fatemeh Sadeghian Nodoushan, and SEYEDHOSSEIN HEKMATI MOGHADDAM, “Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers,” CELL JOURNAL (YAKHTEH), vol. 21, no. 3, pp. 300–306, 2019, [Online]. Available: https://sid.ir/paper/718504/en

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