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Information Journal Paper

Title

Intrauterine xenotransplantation of human Wharton jelly-derived mesenchymal stem cells into the liver of rabbit fetuses: A preliminary study for in vivo expression of the human liver genes

Pages

  89-96

Abstract

 Objective(s): End-stage hepatic failure is a potentially life-threatening condition for which orthotopic Liver transplantation is the only effective treatment. However, a shortage of available donor organs for transplantation each year results in the death of many patients waiting for Liver transplantation. Xenotransplantation, or the transplantation of cells, tissues, or organs between different species, was proposed as a possible solution to the worldwide shortage of Human organs and tissues for transplantation. The purpose of this preliminary study was to reconstruct Human Liver tissue by Xenotransplantation of Human Wharton jelly Mesenchymal stem cells (hWJ-MSCs) into fetal Rabbit. Materials and Methods: Isolation and confirmation of hWJ-MSCs from Human umbilical cord was performed. Eight Rabbits at gestational day 14 were anesthetized. All Rabbits carried pregnancies to term yielding 40 Rabbit fetuses. Intrauterine injection of hWJ-MSCs was performed in 24 fetuses. Twenty-seven fetuses were born alive. Ten Liver samples from injected fetuses were sampled, eight Rabbits 3 days after birth and two Rabbits 21 days after birth. The non-injected fetuses served as positive control. Fetuses of non-injected Rabbits were negative controls. Using real-time polymerase chain reaction (RT-PCR), mRNA expression of albumin (ALB), α-fetoprotein (AFP), hepatic nuclear factor 4 (HNF4), and CYP2B6 (CYP) were detected in Liver samples. Results: The Human ALB, AFP, HNF4, and CYP mRNAs were expressed in the injected sampled fetuses by hWJ-MSCs into fetuses of Rabbits in utero. Conclusion: Developing Xenotransplantation of hWJ-MSCs into Rabbit uterus can introduce an applied approach for producing Human Liver tissue in Rabbits.

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    APA: Copy

    Rezaeian, Leila, HOSSEINI, SEYED EBRAHIM, DIANATPOUR, MEHDI, EDALATMANESH, MOHAMMAD AMIN, TANIDEH, NADER, Mogheiseh, Asghar, & TAMADON, AMIN. (2018). Intrauterine xenotransplantation of human Wharton jelly-derived mesenchymal stem cells into the liver of rabbit fetuses: A preliminary study for in vivo expression of the human liver genes. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(1), 89-96. SID. https://sid.ir/paper/734294/en

    Vancouver: Copy

    Rezaeian Leila, HOSSEINI SEYED EBRAHIM, DIANATPOUR MEHDI, EDALATMANESH MOHAMMAD AMIN, TANIDEH NADER, Mogheiseh Asghar, TAMADON AMIN. Intrauterine xenotransplantation of human Wharton jelly-derived mesenchymal stem cells into the liver of rabbit fetuses: A preliminary study for in vivo expression of the human liver genes. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(1):89-96. Available from: https://sid.ir/paper/734294/en

    IEEE: Copy

    Leila Rezaeian, SEYED EBRAHIM HOSSEINI, MEHDI DIANATPOUR, MOHAMMAD AMIN EDALATMANESH, NADER TANIDEH, Asghar Mogheiseh, and AMIN TAMADON, “Intrauterine xenotransplantation of human Wharton jelly-derived mesenchymal stem cells into the liver of rabbit fetuses: A preliminary study for in vivo expression of the human liver genes,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 1, pp. 89–96, 2018, [Online]. Available: https://sid.ir/paper/734294/en

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