BIOPHYSICAL PROPERTIES OF SINGLE POTASSIUM CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE OF MALE RAT WITH ALZHEIMER’S DISEASE

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JAFARI ADELEH; FAHANIK BABAEI JAVAD; ELIASSI AFSANEH; SAGHIRI REZA

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Journal: Physiology and Pharmacology Year:2013 | Volume:17 | Issue:1 Page(s): 2-14

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Title

BIOPHYSICAL PROPERTIES OF SINGLE POTASSIUM CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE OF MALE RAT WITH ALZHEIMER’S DISEASE

Author(s)

JAFARI ADELEH | FAHANIK BABAEI JAVAD | ELIASSI AFSANEH | SAGHIRI REZA | Issue Writer Certificate

Keywords

ALZHEIMER’S DISEASEQ3

POTASSIUM CHANNELQ3

MITOCHONDRIAQ2

LIPID BILAYERSQ2

Abstract

Introduction: ALZHEIMER’S DISEASE is a progressive neurodegenerative disorder, characterized by impairment of memory and changes in behavior and personality. Recent evidence suggests that MITOCHONDRIAl channels play important roles in memory disorders. Accordingly, the biophysical properties of a single POTASSIUM CHANNEL were investigated in the brain MITOCHONDRIAl inner membrane of rat with ALZHEIMER’S DISEASE.Methods: In the male Wistar rats (220-250 g), ALZHEIMER’S DISEASE was induced by intracerebroventricular injection of amyloid beta 1-42 (4mg/mL). After two weeks, the brain MITOCHONDRIAl inner membranes were extracted. Vesicles were incorporated into lipid bilayer membranes, and single POTASSIUM CHANNEL properties were investigated. Also, purity of the cell fraction was tested by Western blotting. Protein samples were probed with specific antibodies.Results: Based on our previous data, MITOCHONDRIAl inner membrane has a POTASSIUM CHANNEL with a main conductance 93 pS which was 4-AP sensitive and voltage-insensitive at -50 to+40 mV. In the present study, it was demonstrated that the channel conductance was increased to 114 pS in ALZHEIMER’S DISEASE. In addition, the currentvoltage relationship showed an inward rectification. Western blotting and antibodies directed against various cellular proteins revealed that the extracted material contains only MITOCHONDRIA.Conclusion: Our data showed that the biophysical properties (gating, conductance and activities) of POTASSIUM CHANNEL were significantly altered in ALZHEIMER’S DISEASE. Based on these findings, we propose that the brain MITOCHONDRIAl POTASSIUM CHANNELs are involved in ALZHEIMER’S DISEASE, and it can be considered as a target for therapeutic plans.

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APA: Copy

JAFARI, ADELEH, FAHANIK BABAEI, JAVAD, ELIASSI, AFSANEH, & SAGHIRI, REZA. (2013). BIOPHYSICAL PROPERTIES OF SINGLE POTASSIUM CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE OF MALE RAT WITH ALZHEIMER’S DISEASE. PHYSIOLOGY AND PHARMACOLOGY, 17(1), 2-14. SID. https://sid.ir/paper/75054/en

Vancouver: Copy

JAFARI ADELEH, FAHANIK BABAEI JAVAD, ELIASSI AFSANEH, SAGHIRI REZA. BIOPHYSICAL PROPERTIES OF SINGLE POTASSIUM CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE OF MALE RAT WITH ALZHEIMER’S DISEASE. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2013;17(1):2-14. Available from: https://sid.ir/paper/75054/en

IEEE: Copy

ADELEH JAFARI, JAVAD FAHANIK BABAEI, AFSANEH ELIASSI, and REZA SAGHIRI, “BIOPHYSICAL PROPERTIES OF SINGLE POTASSIUM CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE OF MALE RAT WITH ALZHEIMER’S DISEASE,” PHYSIOLOGY AND PHARMACOLOGY, vol. 17, no. 1, pp. 2–14, 2013, [Online]. Available: https://sid.ir/paper/75054/en

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