STRUCTURAL-FUNCTIONAL STUDIES OF PEPTIDES DERIVED FROM A LONG-CHAIN SNAKE NEUROTOXIN NAJA NAJA OXIANA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NASIRIPOUR A.; RANJBAR B.; NADERIMANESH H.; MEHRNEZHAD F.; SOUFIAN S.; SADEGHI GOUDARZ; KOLAHIAN S.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Physiology and Pharmacology Year:2008 | Volume:12 | Issue:3 Page(s): 209-220

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,373

Download:

Cites:

Information Journal Paper

Title

STRUCTURAL-FUNCTIONAL STUDIES OF PEPTIDES DERIVED FROM A LONG-CHAIN SNAKE NEUROTOXIN NAJA NAJA OXIANA

Author(s)

Keywords

LTPQ3

GABAERGIC NEURONQ3

FAST-SPIKING NEURONQ3

METABOTROPIC GLUTAMATE RECEPTORQ3

VISUAL CORTEXQ3

TRANSGENIC MOUSEQ3

Abstract

Introduction: Today the design and structural characterization of mini-proteins with a compact folded structure provide insight into the complex architecture of proteins and has long been a challenging issue in structural-functional studies. Alpha neurotoxins from snake venom have a distinct folded structure comprised of a disulphide core and three loops or "fingers"; each of these loops are considered as a separate functional domain. Because of the selectivity and specificity of snake alpha neurotoxins, they are ideal candidates for structural-functional studies. Methods: With the assumption that each "loop" in the structure of alpha neurotoxin is able to fold as a structurally independent unit and could possibly have functional properties, we have minimized the structure of a long-chain alpha neurotoxin into 18 and 31 amino acid peptides using solid-phase synthesis and cloning methods, respectively. The molecules are structurally studied using circular dichroism spectroscopy and also in vitro using organ bath apparatus and chick biventer cervicis muscle (CBCM).Results: The 18 and 31-mer peptides form predominant beta structures (b-turnand b-sheet/alphahelix) in aqueous solutions which vary with solvent ionic strength. Data from in vitro and in sitico studies indicate that these minimized structures block the twitches in CBCM with concentrations higher than 0.5 11M; and this effect is absolutely dose dependent. On the other hand, the long-chain alpha neurotoxin completely and irreversibly blocks the CBCM even at 50 nanomolar concentration and both effects are post synaptic.Conclusion: These data support the primary assumption that the peptides derived from the second loop of snake alpha neurotoxin can have a distinct folded structure and also exist as an independent biological unit.

Cites

No record.

References

No record.

Cite

APA: Copy

NASIRIPOUR, A., RANJBAR, B., NADERIMANESH, H., MEHRNEZHAD, F., SOUFIAN, S., SADEGHI, GOUDARZ, & KOLAHIAN, S.. (2008). STRUCTURAL-FUNCTIONAL STUDIES OF PEPTIDES DERIVED FROM A LONG-CHAIN SNAKE NEUROTOXIN NAJA NAJA OXIANA. PHYSIOLOGY AND PHARMACOLOGY, 12(3), 209-220. SID. https://sid.ir/paper/75235/en

Vancouver: Copy

NASIRIPOUR A., RANJBAR B., NADERIMANESH H., MEHRNEZHAD F., SOUFIAN S., SADEGHI GOUDARZ, KOLAHIAN S.. STRUCTURAL-FUNCTIONAL STUDIES OF PEPTIDES DERIVED FROM A LONG-CHAIN SNAKE NEUROTOXIN NAJA NAJA OXIANA. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2008;12(3):209-220. Available from: https://sid.ir/paper/75235/en

IEEE: Copy

A. NASIRIPOUR, B. RANJBAR, H. NADERIMANESH, F. MEHRNEZHAD, S. SOUFIAN, GOUDARZ SADEGHI, and S. KOLAHIAN, “STRUCTURAL-FUNCTIONAL STUDIES OF PEPTIDES DERIVED FROM A LONG-CHAIN SNAKE NEUROTOXIN NAJA NAJA OXIANA,” PHYSIOLOGY AND PHARMACOLOGY, vol. 12, no. 3, pp. 209–220, 2008, [Online]. Available: https://sid.ir/paper/75235/en

Related Journal Papers