THE EFFECT OF INSULIN-LIKE GROWTH FACTOR-1, CYSTEAMINE AND β-MERCAPTOETHANOL ON THE IN VITRO MATURATION OF IMMATURE MICE OOCYTES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DEHGHAN MANSHADI A.; ROSTAMI HOSSEINKHANI M.; NIKSERESHT M.; HEDAYATIPOOR A.; AKBAR TABAR TOURI M.; MEHRABI S.; MAHMOUDI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Armaghane Danesh Year:2015 | Volume:20 | Issue:8 (103) Page(s): 720-731

Download Full-Text

Persian Verion

View:

691

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF INSULIN-LIKE GROWTH FACTOR-1, CYSTEAMINE AND β-MERCAPTOETHANOL ON THE IN VITRO MATURATION OF IMMATURE MICE OOCYTES

Author(s)

Keywords

GERMINAL VESICLE OOCYTESQ2

INSULIN-LIKE GROWTH FACTOR 1Q2

CYSTEAMINEQ2

BETA-MERCAPTOETHANOLQ2

CUMULUS CELLQ2

IN VITRO MATURATIONQ2

Abstract

Background & aim: IN VITRO MATURATION of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant CYSTEAMINE and b-Mercaptoethanol on maturation and fertilization of immature oocytes.Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the GERMINAL VESICLE OOCYTES from ovaries were removed and transferred to TCM199 culture medium containing 50 ng/ml insulin-like growth factor-1 and 100 mmol CYSTEAMINE and b-Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test.Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with CUMULUS CELLs in TCM199 medium containing insulin-like growth factor-1, CYSTEAMINE and BME were 92.10, 93.30, 80.60% and in the GV oocytes without CUMULUS CELLs were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P<0.001).Conclusion: In the present study, the simultaneous combination of insulin-like growth factor-1, b-Mercaptoethanol and CYS increased maturation, fertilization and developmental rate to 2-cells stage with CUMULUS CELLs more than the oocyte without CUMULUS CELLs to a greater extent. This represented the need of adding supplemental growth factors and antioxidants to the medium and is associated with CUMULUS CELLs.

Cites

No record.

References

No record.

Cite

APA: Copy

DEHGHAN MANSHADI, A., ROSTAMI HOSSEINKHANI, M., NIKSERESHT, M., HEDAYATIPOOR, A., AKBAR TABAR TOURI, M., MEHRABI, S., & MAHMOUDI, R.. (2015). THE EFFECT OF INSULIN-LIKE GROWTH FACTOR-1, CYSTEAMINE AND β-MERCAPTOETHANOL ON THE IN VITRO MATURATION OF IMMATURE MICE OOCYTES. ARMAGHAN DANESH, 20(8 (103)), 720-731. SID. https://sid.ir/paper/78177/en

Vancouver: Copy

DEHGHAN MANSHADI A., ROSTAMI HOSSEINKHANI M., NIKSERESHT M., HEDAYATIPOOR A., AKBAR TABAR TOURI M., MEHRABI S., MAHMOUDI R.. THE EFFECT OF INSULIN-LIKE GROWTH FACTOR-1, CYSTEAMINE AND β-MERCAPTOETHANOL ON THE IN VITRO MATURATION OF IMMATURE MICE OOCYTES. ARMAGHAN DANESH[Internet]. 2015;20(8 (103)):720-731. Available from: https://sid.ir/paper/78177/en

IEEE: Copy

A. DEHGHAN MANSHADI, M. ROSTAMI HOSSEINKHANI, M. NIKSERESHT, A. HEDAYATIPOOR, M. AKBAR TABAR TOURI, S. MEHRABI, and R. MAHMOUDI, “THE EFFECT OF INSULIN-LIKE GROWTH FACTOR-1, CYSTEAMINE AND β-MERCAPTOETHANOL ON THE IN VITRO MATURATION OF IMMATURE MICE OOCYTES,” ARMAGHAN DANESH, vol. 20, no. 8 (103), pp. 720–731, 2015, [Online]. Available: https://sid.ir/paper/78177/en

Related Journal Papers