EXPRESSION OF OCT-4, SOX-2, NANOG, NUCLEOSTEMIN, BMI-1 AND SOX-9 SELF RENEWAL GENES IN HUMAN UMBILICAL CORD VEIN MESENCHYMAL STEM CELLS

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JAFARI KERMANI A.; FATHI F.; MOULA SEYED JAVAD; GHEYSARI Y.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2008 | Volume:4 | Issue:4 Page(s): 275-284

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Title

EXPRESSION OF OCT-4, SOX-2, NANOG, NUCLEOSTEMIN, BMI-1 AND SOX-9 SELF RENEWAL GENES IN HUMAN UMBILICAL CORD VEIN MESENCHYMAL STEM CELLS

Author(s)

JAFARI KERMANI A. | FATHI F. | MOULA SEYED JAVAD | GHEYSARI Y. | Issue Writer Certificate

Keywords

MESENCHYMAL STEM CELLSQ4

UMBILICAL CORDQ3

PCRQ4

IMMUNOCYTOCHEMISTRYQ3

Abstract

Background and Objectives: Stem cells are defined with two main characteristics which are self renewal potential and pluripotency. It is of great importance to study expression of self renewal genes in different stem cells, because self renewal regulatory mechanisms may vary among different kinds of stem cell. Human UMBILICAL CORD stem cells have recently gained so much attention, because their use in cell therapy methods has several advantages. The main objective of the present study was to evaluate the expression of Oct-4, Sox-2, Nanog, Nucleostemin, Bmi-1 and Sox-9 self renewal genes in human UMBILICAL CORD vein MESENCHYMAL STEM CELLS.Materials and Methods: In this experimental study, UMBILICAL CORDs were obtained in sterile condition and carried to the laboratory in HBSS buffer. MESENCHYMAL STEM CELLS were harvested by collagenase treatment and cultured by means of their adhesiveness to plastic surfaces in DMEM medium supplemented with 15% FBS. To confirm the mesenchymal identity of these cells, expression of some surface markers including CD105, CD106, CD54, CD45, HLA-DR, CD166, and CD34 was analysed by means of flowcytometery. Finally, expression of self renewal genes was evaluated by RT-PCR and IMMUNOCYTOCHEMISTRY techniques.Results: Flowcytometry analysis of UBMCs revealed that the cells are positive for CD105 (98%) and negative for CD34 (0%), CD54 (1%), CD45 (0%), CD106 (0%), CD166 (0%), and HLA-DR (0%). Our results also revealed Oct-4, Nanog, Nucleostemin, Bmi-1 and Sox-9 expression in human UMBILICAL CORD vein MESENCHYMAL STEM CELLS, but not Sox-2 self renewal gene expression.Conclusions: The results showed that the mechanism which is known today for Oct-4, Nanog and Sox-2 function is not the only possible mechanism in which these three genes can play role to regulate self renewal potential of stem cells. Obtained results can offer that a combination of regulatory mechanisms which regulate self renewal of adult and embryonic stem cells, may be responsible to regulate self renewal of stem cells.

Cites

IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS IDENTIFIED BY CELLULAR UPTAKE OF INDOCYANINE GREEN

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APA: Copy

JAFARI KERMANI, A., FATHI, F., MOULA, SEYED JAVAD, & GHEYSARI, Y.. (2008). EXPRESSION OF OCT-4, SOX-2, NANOG, NUCLEOSTEMIN, BMI-1 AND SOX-9 SELF RENEWAL GENES IN HUMAN UMBILICAL CORD VEIN MESENCHYMAL STEM CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 4(4), 275-284. SID. https://sid.ir/paper/78317/en

Vancouver: Copy

JAFARI KERMANI A., FATHI F., MOULA SEYED JAVAD, GHEYSARI Y.. EXPRESSION OF OCT-4, SOX-2, NANOG, NUCLEOSTEMIN, BMI-1 AND SOX-9 SELF RENEWAL GENES IN HUMAN UMBILICAL CORD VEIN MESENCHYMAL STEM CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2008;4(4):275-284. Available from: https://sid.ir/paper/78317/en

IEEE: Copy

A. JAFARI KERMANI, F. FATHI, SEYED JAVAD MOULA, and Y. GHEYSARI, “EXPRESSION OF OCT-4, SOX-2, NANOG, NUCLEOSTEMIN, BMI-1 AND SOX-9 SELF RENEWAL GENES IN HUMAN UMBILICAL CORD VEIN MESENCHYMAL STEM CELLS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 4, no. 4, pp. 275–284, 2008, [Online]. Available: https://sid.ir/paper/78317/en

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