IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS IDENTIFIED BY CELLULAR UPTAKE OF INDOCYANINE GREEN

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RAOUFI A.; AMINI ALI; AZADBAKHT MEHRI; ABOUZARI MORTEZA; FATHI FARDIN

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2010 | Volume:7 | Issue:1 (26) Page(s): 34-40

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Title

IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS IDENTIFIED BY CELLULAR UPTAKE OF INDOCYANINE GREEN

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Keywords

CELL DIFFERENTIATIONQ3

UMBILICAL VEINSQ3

MESENCHYMAL STEM CELLSQ2

HEPATOCYTESQ3

INDOCYANINE GREENQ3

Abstract

Background and Objectives: Umbilical cord is one of the major sources of MESENCHYMAL STEM CELLS. In this study, the differentiative potential of human umbilical vein MESENCHYMAL STEM CELLS into hepatocyte-like cells has been investigated by cellular uptake of INDOCYANINE GREEN. Materials and Methods: Human umbilical vein MESENCHYMAL STEM CELLS (UVMSCs) were incubated for 2 weeks in the medium containing hepatocyte growth factor; they were also treated with the medium containing oncostatin M for another 2 weeks. The differentiated cells were analyzed by uptake of INDOCYANINE GREEN (ICG), immunofluorescence analysis, and periodic acid-Schiff (PAS) staining. Results: The differentiating cells showed transition from bipolar fibroblast-like morphology to round or oval shape. Cellular uptake of ICG was detected in differentiated cells. Glycogen storage was determined by PAS staining in differentiated cells which were immunoreactive to albumin.Conclusions: Based on these observations, we can conclude that UVMSCs are able to differentiate into hepatocyte-like cells in vitro and ICG-staining is a useful marker to identify differentiated HEPATOCYTES from human umbilical vein MESENCHYMAL STEM CELLS.

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APA: Copy

RAOUFI, A., AMINI, ALI, AZADBAKHT, MEHRI, ABOUZARI, MORTEZA, & FATHI, FARDIN. (2010). IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS IDENTIFIED BY CELLULAR UPTAKE OF INDOCYANINE GREEN. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 7(1 (26)), 34-40. SID. https://sid.ir/paper/78707/en

Vancouver: Copy

RAOUFI A., AMINI ALI, AZADBAKHT MEHRI, ABOUZARI MORTEZA, FATHI FARDIN. IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS IDENTIFIED BY CELLULAR UPTAKE OF INDOCYANINE GREEN. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2010;7(1 (26)):34-40. Available from: https://sid.ir/paper/78707/en

IEEE: Copy

A. RAOUFI, ALI AMINI, MEHRI AZADBAKHT, MORTEZA ABOUZARI, and FARDIN FATHI, “IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS IDENTIFIED BY CELLULAR UPTAKE OF INDOCYANINE GREEN,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 7, no. 1 (26), pp. 34–40, 2010, [Online]. Available: https://sid.ir/paper/78707/en

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