STUDY OF IN VITRO PROPERTIES OF FIBRIN SEALANT PREPARED FROM SINGLE DONOR PLASMA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASHEMI TEYR A.; AMIRIZADEH N.; ESHGHI P.; ABOU ALGHASEMI HASAN; AMANI MARYAM; JABARI ALI REZA; RANJBARAN R.; MOHAMMADI MOHAMMAD HOSSEIN; HABIBI ROUDKENAR M.; ALI BALAZADEH A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2009 | Volume:6 | Issue:3 (24) Page(s): 181-189

Download Full-Text

Persian Verion

View:

2,546

Download:

Cites:

Information Journal Paper

Title

STUDY OF IN VITRO PROPERTIES OF FIBRIN SEALANT PREPARED FROM SINGLE DONOR PLASMA

Author(s)

Keywords

FIBRIN SEALANTQ3

FIBRINOGENQ2

THROMBINQ3

PLASMAQ3

Abstract

Background and Objectives: FIBRIN SEALANT (FS) is a PLASMA derived product and has hemostatic, sealing and healing properties and is frequently used to reduce blood loss during and after surgery. Blood bank autologous FIBRIN SEALANTs have no risk of transfusion transmitted diseases. The aim of this study was to obtain of THROMBIN and FIBRINOGEN from FFP and study there in vitro properties for preparation of FS.Materials and Methods: FIBRINOGEN was precipitated by use of protamin sulfate. FIBRINOGEN concentration was assayed with an enzyme-linked immunosorbent assay and clotting clauss method; the effect of temperature on FIBRINOGEN precipitation was also evaluated. THROMBIN was prepared by manual method and TPD and its activity was then determined using specific chromogenic substrate spectrophotometric assay. THROMBIN stability at different temperature degrees was evaluated and clotting time was measured. Tensile strength and adhesion strength were evaluated with the tensiometry device. Clot lysis time was determined by a clot solubility test in 5M urea.Results: FIBRINOGEN concentration precipitated with protamin sulfate was measured as being 73±8 mg/ml. The recovery of FIBRINOGEN in cryoprecipitate was 93%. THROMBIN mixed with FIBRINOGEN had clot time of less than 5 seconds. Tensile strength and adhesion strength of FIBRIN SEALANT were 60±8.9 g/cm2 and 55±9 g, respectively. The average activity of the THROMBIN produced manually was 59.6±6.2. Adding anti-fibrinolttic agent to FIBRINOGEN concentrate has no effect on clotting time and tensile strength but it causes the stability improvement of fibrin clot.Conclusions: FIBRINOGEN and THROMBIN prepared in this experiment have appropriate properties for production of FIBRIN SEALANTs.

Cites

No record.

References

No record.

Cite

APA: Copy

HASHEMI TEYR, A., AMIRIZADEH, N., ESHGHI, P., ABOU ALGHASEMI, HASAN, AMANI, MARYAM, JABARI, ALI REZA, RANJBARAN, R., MOHAMMADI, MOHAMMAD HOSSEIN, HABIBI ROUDKENAR, M., & ALI BALAZADEH, A.. (2009). STUDY OF IN VITRO PROPERTIES OF FIBRIN SEALANT PREPARED FROM SINGLE DONOR PLASMA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 6(3 (24)), 181-189. SID. https://sid.ir/paper/78436/en

Vancouver: Copy

HASHEMI TEYR A., AMIRIZADEH N., ESHGHI P., ABOU ALGHASEMI HASAN, AMANI MARYAM, JABARI ALI REZA, RANJBARAN R., MOHAMMADI MOHAMMAD HOSSEIN, HABIBI ROUDKENAR M., ALI BALAZADEH A.. STUDY OF IN VITRO PROPERTIES OF FIBRIN SEALANT PREPARED FROM SINGLE DONOR PLASMA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2009;6(3 (24)):181-189. Available from: https://sid.ir/paper/78436/en

IEEE: Copy

A. HASHEMI TEYR, N. AMIRIZADEH, P. ESHGHI, HASAN ABOU ALGHASEMI, MARYAM AMANI, ALI REZA JABARI, R. RANJBARAN, MOHAMMAD HOSSEIN MOHAMMADI, M. HABIBI ROUDKENAR, and A. ALI BALAZADEH, “STUDY OF IN VITRO PROPERTIES OF FIBRIN SEALANT PREPARED FROM SINGLE DONOR PLASMA,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 6, no. 3 (24), pp. 181–189, 2009, [Online]. Available: https://sid.ir/paper/78436/en

Related Journal Papers