PRODUCTION OF A RECOMBINANT ANTIMICROBIAL DERMASEPTINE B1 PEPTIDE INNICOTIANA TABACUM L. HAIRY ROOTS WITH ANTIBACTERIAL ACTIVITY

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NAZARI Z.; NAZARIAN FIROUZABADI F.; ISMAILI A.; DARVISHNIA M.

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Journal: Yafteh Year:2018 | Volume:20 | Issue:2 (76) Page(s): 103-118

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Title

PRODUCTION OF A RECOMBINANT ANTIMICROBIAL DERMASEPTINE B1 PEPTIDE INNICOTIANA TABACUM L. HAIRY ROOTS WITH ANTIBACTERIAL ACTIVITY

Author(s)

NAZARI Z. | NAZARIAN FIROUZABADI F. | ISMAILI A. | DARVISHNIA M. | Issue Writer Certificate

Keywords

ANTIMICROBIAL PEPTIDESQ1

AGROBACTERIUM RHIZOGENEQ1

DERMASEPTINEQ1

GENE EXPRESSIONQ2

Abstract

Background: Root hair culture is a valuable system to produce recombinant proteins in planta. ANTIMICROBIAL PEPTIDES (AMPs) are vital parts of the innate immune response found in almost allforms of life. Precise target activity and limited toxicity towards mammalian cells make themsuitable candidate molecules to combat evolving drug-resistant microorganisms. The aim of thepresent study was to produce a Dermaseptin B1 recombinant antimicrobial peptide in Nicotianatabacum root hair and assess the antibacterial activity of the protein extract from transgenic roothairs. Materials and Methods: A Dermaseptin B1 encoding gene sequence was C-terminally fused to aChitin Binding Domain (CBD) encoding sequence and cloned in a plant binary vector used forAgrobacterium rhizogenes-mediated transformation to generate root hairs in tobacco. Transgenicroot hairs were produced, and protein extracts were used to assess antimicrobial activity against anumber of microbes. Results: PCR and RT-PCR analysis confirmed the integration of the Dermaseptin B1 gene in a roothair cell genome and the presence of Dermaseptin B1 mRNA transcripts, respectively. Recombinantprotein had a significant (P<0. 05) antibacterial effect towards gram-positive and gram-negativebacteria. Conclusion: DERMASEPTINE B1 recombinant peptide was successfully produced in tobacco root haircells and its antibacterial effects was confirmed. These results suggest that the recombinant proteinmay have a therapeutic effect to control bacterial pathogens. It can be concluded that root hair cellscan be used to produce and purify valuable recombinant proteins with pharmaceutical applications.

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APA: Copy

NAZARI, Z., NAZARIAN FIROUZABADI, F., ISMAILI, A., & DARVISHNIA, M.. (2018). PRODUCTION OF A RECOMBINANT ANTIMICROBIAL DERMASEPTINE B1 PEPTIDE INNICOTIANA TABACUM L. HAIRY ROOTS WITH ANTIBACTERIAL ACTIVITY. YAFTEH, 20(2 (76) ), 103-118. SID. https://sid.ir/paper/80261/en

Vancouver: Copy

NAZARI Z., NAZARIAN FIROUZABADI F., ISMAILI A., DARVISHNIA M.. PRODUCTION OF A RECOMBINANT ANTIMICROBIAL DERMASEPTINE B1 PEPTIDE INNICOTIANA TABACUM L. HAIRY ROOTS WITH ANTIBACTERIAL ACTIVITY. YAFTEH[Internet]. 2018;20(2 (76) ):103-118. Available from: https://sid.ir/paper/80261/en

IEEE: Copy

Z. NAZARI, F. NAZARIAN FIROUZABADI, A. ISMAILI, and M. DARVISHNIA, “PRODUCTION OF A RECOMBINANT ANTIMICROBIAL DERMASEPTINE B1 PEPTIDE INNICOTIANA TABACUM L. HAIRY ROOTS WITH ANTIBACTERIAL ACTIVITY,” YAFTEH, vol. 20, no. 2 (76) , pp. 103–118, 2018, [Online]. Available: https://sid.ir/paper/80261/en

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