Introduction: Head lice infection caused by pediculus and its treatment is a world wide and historical concern. Methods: This study has been carried out in different towns of Hamadan province in 2000-2001.Screening was done in 120 girls elementary schools during which 729 students were examined for head lice infection. Finally about 13.5% (975) of the girls were detected with head lice. The infected girls were assigned into three treatment groups with three kinds of shampoos against head lice. The participants assigned to the group used the shampoos on manufacture's instruction and the results were considered in three weeks after the treatment. Statistical analyses were performed by X2 test and line logarithmic procedure.Results: The results showed that the effect (no live adult and nymph of lice were seen on physical reexamination of sclap), at the end of the first week were 29,36,34 and 15 percentage for dphenothrin 0.2%, lindane 1%, permethrin and 1% placebo respectively. These cure rates for treatment shampoos show the effectiveness of them
compared to placebo (0.001 < pvalue<0.01). Also at this time, the efficacy of dphenothrin 0.2% (29%) compared to lindane 1% (36%) is less (pvalue<0.05). In the 2nd week, these results effectively were 72%, 72% and 71% for d-phenothrin 0.2%, lindane and 1% permethrin respectively, Which show no difference with each other (pvalue>0.05). However, at the end of third week, the cure rates for the three mentioned shampoos were 88, 88 and 87 percentage and also there is a shampoo just for the first week after usage. Line logarithmic analysis of results shows that efficacy of different pediculicide shampoo(pvalue=0.58) in different times (pvalue=0.25) makes no difference. But the cure rate of cases varied in three weeks after treatment (pvalue<9.0001). This Variety proves the residual effect of pediculicides on new hatched nymphs. Conclusion: According to the results obtained as well as considering economic viewpoints, and popular conclusion, shampoo lindane 1% is advocated as the first choice treatment of head lice infestation, if is applied under the manufacturer's instruction and with caution combined with physical removal of nits with fine-toothed comb. Finally, educative campaign of the parents and the school staff is recommended.