MINIMIZING THE NON ALLERGIC MATERIAL IN SAFFRON POLLEN EXTRACT BY CHANGING THE CONDITIONS OF EXTRACTING TECHNIQUE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAVALLAEE SH.; SANKIAN M.; KAGHAZIAN H.; VARASTEH A.R.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Birjand University of Medical Sciences Year:2000 | Volume:7 | Issue:1 (12) Page(s): 18-21

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

959

Download:

Cites:

Information Journal Paper

Title

MINIMIZING THE NON ALLERGIC MATERIAL IN SAFFRON POLLEN EXTRACT BY CHANGING THE CONDITIONS OF EXTRACTING TECHNIQUE

Author(s)

TAVALLAEE SH. | SANKIAN M. | KAGHAZIAN H. | VARASTEH A.R. | Issue Writer Certificate

Keywords

ALLERGENQ3

SAFFRON POLLENQ4

SDS-PAGEQ3

Abstract

Introduction and aim: More than 20% of people are suffering from etopic allergic disease. For diagnostic tests and desensitization of allergic patients, the raw extract of ALLERGENs is used. However, these extract usually contain more than 50 different kinds of ALLERGENs along non-ALLERGENs protein molecules which causes difficulties for the use of extracts in diagnostic tests. The aim of this study was to produce a procedure that could extract all ALLERGENs from plants pollens while minimizing the amount non-ALLERGENic material in extracts.Methods: Factors such a PH, morality of buffer, dialysis and shaking in comparison to mixing with the use of magnet in the SDS-PAGE extracting technique were studied. The composition of extracts by SDS-PAGE technique and protein measurement by Bradford technic were evaluated.Results: The SDS-PAGE protein extract, showed 6 bands in the range from 14 to 94 KD of relative molecular weights. Oialysis of extracts decreased background colore intensity and made the extracted protein bands sharper, especially in the region lower than 30 KD. In comparison with mixing, sampling by shaking method at 24 and 48 hours gave sharper protein bands.Conclusion: This study showed that changing the conditions during pollen extracting may neutralize the effects of agents that destroy the ALLERGENs.

Cites

No record.

References

No record.

Cite

APA: Copy

TAVALLAEE, SH., SANKIAN, M., KAGHAZIAN, H., & VARASTEH, A.R.. (2000). MINIMIZING THE NON ALLERGIC MATERIAL IN SAFFRON POLLEN EXTRACT BY CHANGING THE CONDITIONS OF EXTRACTING TECHNIQUE. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, 7(1 (12)), 18-21. SID. https://sid.ir/paper/95356/en

Vancouver: Copy

TAVALLAEE SH., SANKIAN M., KAGHAZIAN H., VARASTEH A.R.. MINIMIZING THE NON ALLERGIC MATERIAL IN SAFFRON POLLEN EXTRACT BY CHANGING THE CONDITIONS OF EXTRACTING TECHNIQUE. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2000;7(1 (12)):18-21. Available from: https://sid.ir/paper/95356/en

IEEE: Copy

SH. TAVALLAEE, M. SANKIAN, H. KAGHAZIAN, and A.R. VARASTEH, “MINIMIZING THE NON ALLERGIC MATERIAL IN SAFFRON POLLEN EXTRACT BY CHANGING THE CONDITIONS OF EXTRACTING TECHNIQUE,” JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, vol. 7, no. 1 (12), pp. 18–21, 2000, [Online]. Available: https://sid.ir/paper/95356/en

Related Journal Papers