Effects of nutrients and light density on the growth performance and and density of micro-algae Senedesmus sp in different fomula were studied in orther to determination of higher density in minimum period. The TMRL media with different concentrations of N (N2: 30, 50, 70g), P (NaH2Po4: 3, 5, 7g) and same content of Fe (FeCl3: 1g) were prepared. All treatments have been stocking with 1725×103 Cell/ml or 6. 23 Log C/ml. Micro-algae was cultured into these media (M) with two light density (L) as 1 (3500 ± 350 Lux) and 2 (5500 ± 350 Lux) in 6 treatments and 3 replications during 12 days. At the days of 2 and 12, algae biomass were 6. 24 and 7. 05 Log C/ml in M1-L1, 6. 25 and 7. 42 Log C/ml in M2-L1 and 6. 25 and 7. 41 in M3-L1, respectively. Algae biomass at the days of 2 and 12, were 5. 65 and 7. 04 Log C/ml in M1-L2, 6. 18 and 7. 35 Log C/ml in M2-L2 and 6. 35 and 7. 43 in M3-L2, respectively. The results showed that growth of Scenedesmus in the third medium (M3 contained P-7g, N-70gr and Fe-1g) and fisrt light density (L1: 3500 Lux) after 12 days of culture was much higher than respect to the rest of media. Thus, growth prformance of Scenedesmus was higher in media which had high contents of nutrinet (P<0. 05). The results also showed that amounts of protein, lipid, carbohydrate and ash based dry weight in micro-algae Scenedesmus were 32. 4, 5. 21, 24. 12 and 32. 55 percent, respectively.