One predicament in present organizations is the existence of anti-citizenship behaviors such as laziness, aggression, bullying, stubbornness, intimidation, revengefulness. These behaviors affect both on organizations' performance and interpersonal relationships and employees cooperation spirit. The main goal of this research was to identify the effect of work alienation on incidents anti-citizenship behaviors through the mediating role of job burnout. This study was an applied research method based on structural equation modeling. The population of the research was all the managers and assistances of the first and second high schools of Zahedan in the academic year of 2018-19. 252 principals and assistants of Zahedan high schools were studied by stratified random sampling method. To collect information, three questionnaires were used: Anti-Citizenship Behaviors (Rahmani, 2013), Job Burnout (Maslach & Jackson, 1981) and Work Alienation (adopted from Sadaghati Fard and Abdollahzadeh, 2009). For data analysis the correlation coefficient and structural equation modeling were used by SPSS16 and Lisrel. Based on the results work alienation, either directly or indirectly through mediation of job burnout, had a positive effect on the appearing anti-citizenship behaviors. Therefore, measures such as establishing a system of suggestions, delegation of authority, diversity job and contributing employees to decision-making, while reducing work alienation, could reduce job burnout and anti-citizenship behaviors.