With respect to widely usage of new neuroimaging devices (such as MRI), brain lesions are revealed in primary phases, where definite diagnosis in this phase could lead to employing necessary therapies which in turn prevent their progression to untreatable phases. For definite diagnosis, sampling with stereotactic procedure is strongly recommended. A study conducting during 1990-2000 in Shohada-e- Tajrish Hospital had surveyed 90 brain lesions with definite spectrum of infectious, tumoral and demyelinating. This study has shown different types of these lesions with their related prevalence. Among these patients, 60 were male (66.6%) and 30 were female (33.3%), where mean age was 42.1 years old. Pathologic prevalence of different brain lesions were as below: Lymphoma, which had ascending movement, comprises 30 cases (33.3%), metastatic lesions 20 cases (22.2%), glial tumors 14 cases (15.5%), brain abscess 6 cases (6.66%), non specific pathologic feature 5 cases (5.5%), gliosis 4 cases (4.4%) and 11 cases (12.2%) of other rare lesions such as germinomas, tuberculomas, leuko encephalopathy, infarction and demyelinating lesions. Different brain lesions comprise a wide specirum of disease, each require specific therapeutic approach whereas obtaining biopsy and the pathologic features of these lesions could lead to better therapeutic outcomes.