In the present study, 47 advanced wheat lines for dryland areas were evaluated for resistance to different pathotypes of yellow rust at seedling and adult-plant stages. Pathotypes 6E14A-, 38E18A +, 134E134A+, and 134E150A+ were used in the greenhouse (seedling stage) tests. Field tests were conducted in Karaj, Sari, Moghan, Islamabad, Orumieh, Khoramabad, Boroujerd, Mashhad, Sanandaj, and Gachsaran Research Stations. In addition to planting susceptible check, Bolani, after each 20 entries, it was planted around the experiment as spreader. Mist irrigation systems were used in Karaj, Mashhad, Orumieh, Islamabad, and Moghan Research Stations and artificial inoculation were done in Karaj and Moghan at tillering, flag leaf, and booting stages using spores which were previously collected from these areas. Inoculations. were done in the afternoons. In seedling tests, 0-9 scale was used for recording seedling infection types, and infection types and severity were used for assessment of adult-plant responses. At seedling stage the infection types were increased with increase in virulence factors for Yr-genes in used pathotypes. Varieties Dari 2, Dari 3, Dari 4, Dari 5, and Dari 6 were completely resistant to all used pathotypes, that indicated their resistance may might be controlled at least by one seedling resistance gene(s) which all pathotypes were a virulent for that (those) gene(s). Pathotype 134E134A+ were used in both seedling and adult-plant tests at Karaj experiments and according to the obtained results, entries no: 10, 16, 18, 19, 20, 24, 27, 28, 30, 33, 36, 37, 38, 41, and 42 were susceptible at seedling and resistant at adult-plant. This possibly indicates presence of some adult-plant resistance genes in these varieties.