Background & Objectives: University of Medical Sciences is one of the most important organs of service to the citizens. Its main purpose is to provide services and meet the needs of citizens. The investigation of anti-citizenship behavior of staff and its relationship with the managers’ way of political behavior and, ultimately, the roots of problems, Could provide a better service and deserve to the citizens.Material & Methods: The present study is descriptive (correlation analysis), and in terms of the purpose, it is practical. This study aimed to investigate the role of moderator of the type of personality in the relationship between the managers’ political attitudes and anti-Citizenship’s behaviors of employees of Mashhad University of Medical Sciences. According to statistics in 1393, the total number of employees is 750 people, among which 250 people with formula samples were selected by the researchers. The survey questionnaire was used to collect information. Then, by using SPSS and LISREL software, the data were analyzed by the researchers.Results: Political behavior of the managers increased the anti-Citizenship behaviors of employees. (p=0.000 r=0.275). In addition, types of the personality can adjust the relationship between political attitudes and behavior of managers. P (0.030).Conclusions: The study showed that there is a relationship political behaviors of the managers and anti-Citizenship behaviors of staff. In other words, the political behavior of managers increases anti-Citizenship behaviors of staff. Among the political behavior of managers, dimensions of persuasion and appeal to values, blaming, threatening, supportive base hits and obscure secrecy had the greatest impact on anti-Citizenship behaviors of staff. Moreover, the results showed that the types of personality, as an intervening variable, affect the relationship. And those with type B personality lower than those with type A, engage less to the anticitizenship behaviors.