Introduction: Lack of motivation is one of the most causes of decreased organizational outcome. In spite of applying a verity of proposed motivational issues in work, disinterestedness and job dissatisfaction are visible among nurses. The main goal of this study was to determine the motivation factors and effect of nurses’ Job satisfaction related to patient education in Tabriz hospitals. Methods: This was a Cross-sectional study, and 325 registered nurses were participated in this study. Method of sampling was stratified random sampling. Information gathered through questionnaire. All data gathering for this study was analyzed by using descriptive statistically methods, and also correlation test. Data analysis was accomplished by SPSS. Results: Participants stated that 94% job salary, 94% conditions of job environment, 80% good relationships with colleges, 74% job control and strategy of job management were significant external factors of job motivation in patient education. Also 80% of respondents said that job nature, 77% cognition and appreciating with others, 74% job responsibility, and 68% job development were significant internal factors of job motivation in patient education. Ultimately, nurses believed that 81% external and 72% internal factors were very significant for creation of motivation. Findings showed that 78. 3% of nursing staff were dissatisfied. Conclusion: Professional identity is core variable and acts as axial driving force for nurses job motivation. Nurses acquire their professional identity by obtaining dignity, appropriate organizational structure, sufficient income and facilities, and suitable social status that ultimately resulted in high level of job motivation. This consequence would increase the quality of care, nursing effectiveness, and finally led to high status of individual and community health level.