Introduction: Using e-learning in medical universities grows fast and the quality of its content is consideredan important issue. The aim of this study was to investigate the effectiveness of e-learning content of Facultyof Nursing and Midwifery at Mashhad University of Medical Sciences. Methods: This descriptive study investigated all faculty members of Faculty of Nursing and Midwifery (n= 55) at Mashhad University of Medical Sciences in 2015-2016. Data were collected by a researcher madequestionnaire. To determine the validity of the instrument, content validity was used and the reliability of thequestionnaire with the Cronbach's alpha coefficient for its components was 0. 73, 0. 70, 0. 85, 0. 70, 0. 72 and0. 65, respectively. To analyze the data, descriptive (mean and standard deviation) and inferential statistics(One-Sample T test) were used. Result: The components of "importance", according to 80 percent significantly higher (t (54) =4/90). butother components of the components "interest learner" (t=(54)-4/17), the factor of "usefulness" (t(54)=-321/5)component "ability to learn" (t(249)=17/01), and the components of "flexibility" (t(54)=-1/54, P>0/05), theaverage of the sample mean hypothetical (16) were less significant than harm. Conclusion: Among the components of the quality of e-learning content; "interested learner", "usefulness", "ability to learn" and "flexibility" Not Favorable conditions, and only components of "importance" waseligible.