In order to investigate the effect of water stress at different growth stages on the yield and quality of tomato, a field experiment was conducted at Agricultural Research Center of Semnan (Shahrood), during 1999-2001 years. The split-plot design with subplots in strips and three factors were used. Tomato varieties (Mobile, Cal- j) were trans planted in two strips in each block. Main plots were different amounts of water (50, 75 and 100 percent of full irrigation), and subplots were growth stages of plant (transplanting- flowering, flowering-fruit formation, fruit formation-first harvesting and after first harvesting-end of season). Irrigation requirement was determined by class-A evaporation pan. Irrigation method was furrow irrigation. The results showed that, there was no difference between the yield of the two varieties. Irrigation levels of 75 and 50. (25 and 50 percent water stress) decreased the yield of tomato in the S2 stage (flowering-fructify). This decrease was about 13 ton per hectare (50 percent stress), but water stress had no significant effect on the yield, at other growth stages. Water levels of 50, 75,100 percent with two season average yields of 58.3, 57 and 62 ton per hectare, respectively, were not significantly different. Water stresses affected the pH and BRIX index of the samples. The pH and BRIX increased with increase of water stress, so that the maximum pH and BRIX were obtained from 50% water stress treatment. The pulp to juice ratio decreased with increase of stress. The maximum water use efficiencies were 9.6 and 9.5 (kg/m3ha) for 75% in the 4th growth stage and 50% irrigation in the first growth stage, respectively.