5E teaching method or structuralism is a theory derived from philosophical social movement of postmodernism in the 20th century. This method, is considered as the major five stages to conduct structuralism pattern consisting of engagement, exploration, explanation, extension, and evaluation. The aim of this research is to determine the difference resulted by 5E teaching method in 6 dimensions of cognitive domain (knowledge, understanding, application, analysis, combination and evaluation) of learning of biology lesson. The technique used in it is qwasiexperimental (independent, two-group (experimental and control) with post-test). The statistical universe of this research consists of all ten th grade boy high school students Khalkhal during 96-1395. In order to gather theoretical bases and the literature of this research, documental technique as well as other related studies were used while questionnaire technique (researcher-made written test) was employed to evaluate hypotheses. Regarding the abnormality of data, the non-parametric test of Mann-Whithney was uses and to analyze and evaluate the hypotheses the spss21-statistical software was employed. The results showed, there is a meaningful difference between the control and experimental groups in this regard, by using 5E method. These results show the role and importance of employing 5E in teaching.