Objective: To detect-Salmonella serotypes of the cow's faecal samples by means ompC gene.Design: Experimental study.Samples: Two hundred fea-cal samples (50 samples from Khorasan province, 20 samples from Zanjan province, 30 samples from Amin Abad Institute, 30 samples from, Mardabad Veterinary Clinic and 70 samples from farms –around Tehran province) and 25 samples from Shahr-e-kord.Procedures: The samples were tested by PCR in parallel with conventional culture and serogrouping methods. PCR was carried out after enrichment of faecal samples in selenite- F broth, DNA was extracted by rapid boiling method. PCR was done and products electrophoresed on a 1.5 % agarose gel and visualized by UV transilluminator after staining with ethidium bromide. Visible band at expected size (159 bp) was defined as a positive response.Statistical analysis: Descriptive study.Results: Results showed that in all of 25 isolates and 14 (7%) out of 200 faecal samples, target gene (ompC gene with 159 bp size) was observed. Among them only one sample (0.5%) was both PCR and culture positive. Conclusion: According to the results of this study PCR assay with enrichment broth processing method is useful for rapid identification of Salmonella serotypes for in faecal samples.