This experiment was conducted to investigate the effect of using 2% untreated and treated corn gluten meal with protease enzyme (300 mg/kg) at three different times (120, 180 and 240 minutes) on broiler chicks performance, carcass characteristics and some blood parameters. 240 commercial strains Ross 308 male broiler chicks with five treatments including: 1. control diet, 2. control diet containing 2% corn gluten, 3, 4, and 5. control diet containing 2% corn gluten meal processed with protease enzyme at 120, 180 and 240 minutes, respectively, four replicates and 12 chicks in each replicate in a completely randomized design were reared for 38 days. During the experiment, feed intake, body weight gain and feed conversion ratio were measured. To evaluate blood parameters and carcass components at 38 d, eight birds from each treatment were selected and after blood samples collection, some blood parameters such as glucose, total protein, albumin, globulin, triglycerides, cholesterol, HDL-C, LDL-C and VLDL-C were measured and also, carcass components including weights of carcass, thighs, breast, abdominal fat, liver, pancreas, spleen and Bursa Fabricius were determined. Results of this study indicated that treatments containing 2% corn gluten meal with protease enzyme processing at different times, increased feed intake (3801. 98, 3848. 59 and 3850. 42 vs. control diet 3769/82 g), body weight gain (2000. 88, 2020. 16 and 2033. 27 vs. control diet 1888. 82 g), and reduced feed conversion ratio (1. 90, 1. 90 and 1. 89 vs. control diet 1. 99) in total period of the experiment (P<0. 05). In this experiment, the experimental diets had not significant effect on carcass components and blood parameters. In general, the results of the present experiment showed that the use of treated corn gluten meal with protease enzyme at different times improved performance in the diet of broiler chicks.