Introduction: Human flourishing is one of the important issues that have been currently the focus of positive psychology. Therefore, the study of flourishing and related variables can be helpful in reducing anxiety, stress, and depression. According, this study aimed to evaluate the effectiveness of flourishing education on anxiety, stress, and depression in students of Babol University of Medical Sciences. Materials and Methods: The present study was an applied study that employed a quasi-experimental design with pretest-posttest and a control group. The research population included all students of Babol University of Medical Sciences. A total of 38 students who scored a standard deviation lower than the mean in the flourishing questionnaire were selected by random cluster sampling and divided into two groups. Before, the intervention, the participants in the two groups completed the Anxiety, Stress and Depression Inventories. Then, the participants in the intervention group attending the flourish training program. Finally, both groups took the post-test. The data were analyzed using multivariate analysis of covariance (MANCOVA). Results: The results of multivariate analysis of covariance (MANCOVA) showed that the two groups had significant differences in terms of anxiety (F=10. 983, P=0. 002), stress (F=9. 174, P=0. 005), and depression (F=4. 252, P=0. 047) after the intervention. This suggests that flourishing training reduced anxiety, stress and depression experienced by the participants in the intervention group. Conclusion: In line with the findings of this study, it can be suggested that implementing flourishing training reduces students' stress, anxiety, and depression.