PURIFICATION OF BLOOD COAGULATION FACTOR V ACTIVATOR FROM THE VENOM OF IRANIAN VIPERA LEBETINA

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AMOOZEGARI ZOHREH; ZARE MIRAK ABADI ABBAS; NOORBEHBAHANI MOZHGAN

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Journal: Jundishapur Scientific Medical Journal Year:2013 | Volume:12 | Issue:1 (82) Page(s): 21-32

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Title

PURIFICATION OF BLOOD COAGULATION FACTOR V ACTIVATOR FROM THE VENOM OF IRANIAN VIPERA LEBETINA

Author(s)

AMOOZEGARI ZOHREH | ZARE MIRAK ABADI ABBAS | NOORBEHBAHANI MOZHGAN | Issue Writer Certificate

Keywords

FACTOR V ACTIVITYQ1

SNAKE VENOMQ2

VIPERA LEBETINAQ2

Abstract

Background and Objective: The venom of many Viperidae snake appear to contain proteins that affect blood coagulation. The aim of this study was to identify and isolate a blood coagulation factor V activator present in the venom from VIPERA LEBETINA.Material and Methods: Factor V activator was purified from 200 mg of crude venom by three steps of chromatorgraphy which included: gel filtration on sephadex G100, Ion exchange chromatography on DEAE and cellulose and affinity chromatography on heparin agarose. The effect of factor V activator on human factor V was studied by measuring the amidiolytic activity of the produced thrombin by activated factor V (Va).Results: The results of SDS – PAGE identified an activator of factor V as a single protein band with a molecular weight of 29 KDa. This compound was converted, in the presence of calcium ions, to active factor Va. It also had arginine esterase activity toward substrate BAEE (NX-benzoyl arginine ethyester) and a weak amidase activity on S-2222 (benzoylle-glu-Gly-Arg-p- nitroanilide).Conclusion: The results of this study showed that the isolated factor is a specific activator on factor V.

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APA: Copy

AMOOZEGARI, ZOHREH, ZARE MIRAK ABADI, ABBAS, & NOORBEHBAHANI, MOZHGAN. (2013). PURIFICATION OF BLOOD COAGULATION FACTOR V ACTIVATOR FROM THE VENOM OF IRANIAN VIPERA LEBETINA. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 12(1 (82)), 21-32. SID. https://sid.ir/paper/12412/en

Vancouver: Copy

AMOOZEGARI ZOHREH, ZARE MIRAK ABADI ABBAS, NOORBEHBAHANI MOZHGAN. PURIFICATION OF BLOOD COAGULATION FACTOR V ACTIVATOR FROM THE VENOM OF IRANIAN VIPERA LEBETINA. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2013;12(1 (82)):21-32. Available from: https://sid.ir/paper/12412/en

IEEE: Copy

ZOHREH AMOOZEGARI, ABBAS ZARE MIRAK ABADI, and MOZHGAN NOORBEHBAHANI, “PURIFICATION OF BLOOD COAGULATION FACTOR V ACTIVATOR FROM THE VENOM OF IRANIAN VIPERA LEBETINA,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 12, no. 1 (82), pp. 21–32, 2013, [Online]. Available: https://sid.ir/paper/12412/en

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