MEASUREMENT OF HYALURONIDASE ENZYME ACTIVITY IN VENOM OF IRANIAN VIPERA LEBETINA

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AMOOZEGARI ZOHREH; ABYAZ SAMANEH; NOORBEHBAHANI MOZHGAN

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Journal: Jundishapur Scientific Medical Journal Year:2016 | Volume:15 | Issue:3 Page(s): 363-370

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Title

MEASUREMENT OF HYALURONIDASE ENZYME ACTIVITY IN VENOM OF IRANIAN VIPERA LEBETINA

Author(s)

AMOOZEGARI ZOHREH | ABYAZ SAMANEH | NOORBEHBAHANI MOZHGAN | Issue Writer Certificate

Keywords

VIPERA LEBETINAQ1

VENOMQ2

HYALURONIDASEQ2

Abstract

Background and Objective: Snake VENOMs comprise complex mixtures of enzymatic and non-enzymatic proteins and small organic compounds. HYALURONIDASE is a constant factor in the VENOM of the snake and is known as a spreading factor. This factor facilitate spread of toxins that harm into the blood circulation system.In this paper, we describe the determination of HYALURONIDASE activity in Iranian VIPERA LEBETINA VENOM.Subjects and Methods: HYALURONIDASE activity was assayed turbidometrically on hyaluronic acid. Turbidity reducing activity was expressed as a percentage of the hydrolysed hyaluronate, taking the absorbance of a tube as 100% in which no enzyme was added. Bovin testis HYALURONIDASE was used as standard.Results: The optima PH and temperature for HYALURONIDASE maximum activity was 6 and 37oC respectively.Conclusion: In conclusion the Iranian VIPERA LEBETINA VENOM possessed considerable HYALURONIDASE activity.

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APA: Copy

AMOOZEGARI, ZOHREH, ABYAZ, SAMANEH, & NOORBEHBAHANI, MOZHGAN. (2016). MEASUREMENT OF HYALURONIDASE ENZYME ACTIVITY IN VENOM OF IRANIAN VIPERA LEBETINA. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 15(3), 363-370. SID. https://sid.ir/paper/12556/en

Vancouver: Copy

AMOOZEGARI ZOHREH, ABYAZ SAMANEH, NOORBEHBAHANI MOZHGAN. MEASUREMENT OF HYALURONIDASE ENZYME ACTIVITY IN VENOM OF IRANIAN VIPERA LEBETINA. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2016;15(3):363-370. Available from: https://sid.ir/paper/12556/en

IEEE: Copy

ZOHREH AMOOZEGARI, SAMANEH ABYAZ, and MOZHGAN NOORBEHBAHANI, “MEASUREMENT OF HYALURONIDASE ENZYME ACTIVITY IN VENOM OF IRANIAN VIPERA LEBETINA,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 15, no. 3, pp. 363–370, 2016, [Online]. Available: https://sid.ir/paper/12556/en

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