IN VITRO IMMUNOMODULATORY EFFECT OF R10 FRACTION OF GARLIC ON VIABILITY AND PRODUCTION OF TNF-Α IN CD8+ T CELLS

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GHAZANFARI T.; RASHIDI H.; JALAEI SH.; ALIJANI P.

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Journal: Avicenna Journal of Clinical Medicine Year:2014 | Volume:20 | Issue:4 (SN 70) Page(s): 273-279

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Title

IN VITRO IMMUNOMODULATORY EFFECT OF R10 FRACTION OF GARLIC ON VIABILITY AND PRODUCTION OF TNF-Α IN CD8+ T CELLS

Author(s)

GHAZANFARI T. | RASHIDI H. | JALAEI SH. | ALIJANI P. | Issue Writer Certificate

Keywords

CD8+ CELLSQ2

CYTOKINESQ2

GARLICQ3

R10 FRACTIONQ2

VIABILITYQ2

Abstract

Introduction & Objective: -cells, especially CD8+ T lymphocytes are the most important cells in anti-tumor response. Previously R10 FRACTION of GARLIC extract was reported as an immuno-modulator which induced an effective cellular immunity and Th1 responses. In this study the in vitro immuno-modulatory effect of R10 on CD8+ T cells VIABILITY and production of TNF-a were evaluated.Materials & Methods: In this experimental study, using monoclonal antibodies attached to magnetic beads with isolating columns by magnetic bead method, CD8+ T cells from spleen cells of Balb/C mice were isolated. R10 FRACTION based on molecular weight was prepared using Ultra filtration. MTT assay was used to evaluate cell VIABILITY. TNF-a level was measured in the supernatant of culture of CD8+ T cells by ELISA. Obtained data was compared and analyzed using Nonparametric Test and Keraskel & Wanny's Test tests.Results: The findings indicate that all dilutions of R10 FRACTION increased cell VIABILITY of CD8+ T cells in comparison with the negative control group and in the presence of ConA with dilution of 1:50 of R10 FRACTION significantly increased cell VIABILITY of CD8+ T Cells compared to ConA alone. Secretion of TNF-a significantly increased by all dilutions of R10 FRACTION.Conclusion: These findings suggest that R10 FRACTION of GARLIC can be used as an Immuno-modulator drug candidate for induction of cellular Immunity in tumor therapy.

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APA: Copy

GHAZANFARI, T., RASHIDI, H., JALAEI, SH., & ALIJANI, P.. (2014). IN VITRO IMMUNOMODULATORY EFFECT OF R10 FRACTION OF GARLIC ON VIABILITY AND PRODUCTION OF TNF-Α IN CD8⁺ T CELLS. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 20(4 (SN 70)), 273-279. SID. https://sid.ir/paper/17754/en

Vancouver: Copy

GHAZANFARI T., RASHIDI H., JALAEI SH., ALIJANI P.. IN VITRO IMMUNOMODULATORY EFFECT OF R10 FRACTION OF GARLIC ON VIABILITY AND PRODUCTION OF TNF-Α IN CD8⁺ T CELLS. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2014;20(4 (SN 70)):273-279. Available from: https://sid.ir/paper/17754/en

IEEE: Copy

T. GHAZANFARI, H. RASHIDI, SH. JALAEI, and P. ALIJANI, “IN VITRO IMMUNOMODULATORY EFFECT OF R10 FRACTION OF GARLIC ON VIABILITY AND PRODUCTION OF TNF-Α IN CD8⁺ T CELLS,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 20, no. 4 (SN 70), pp. 273–279, 2014, [Online]. Available: https://sid.ir/paper/17754/en

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