COMPARISON OF TWO COMMERCIAL DERMAL MATRIX ON BIOLOGICAL BEHAVIOR OF ENDOTHELIAL CELLS(IN VITRO)

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MAGHADAS OMID; JAFARI EHSAN; BAKHSHI BORHAN; HARANDI MOHAMAD

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Journal: JOURNAL OF RESEARCH IN DENTAL SCIENCES Year:2019 | Volume:15 | Issue:4 (58) Page(s): 218-226

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Title

COMPARISON OF TWO COMMERCIAL DERMAL MATRIX ON BIOLOGICAL BEHAVIOR OF ENDOTHELIAL CELLS(IN VITRO)

Author(s)

MAGHADAS OMID | JAFARI EHSAN | BAKHSHI BORHAN | HARANDI MOHAMAD | Issue Writer Certificate

Keywords

DERMAL CELLSQ1

MATRIXQ1

ENDOTHELIAL CELLQ1

BIOLOGICAL BEHAVIORQ1

Abstract

Aim and background: Behavior of ENDOTHELIAL CELLs can have significant role regarding theirproliferation and attachment on acellualr dermal grafts in regeneration procedures. The purpose of thisstudy is to evaluate the effects of two dermal scaffolds on attachment and proliferation of humanumbilical vein ENDOTHELIAL CELLs. Methods and Materials: This study was conducted on experimental approach. Human umbilical veinendothelial cells were seeded, experimentally. Two commercially available types of ADM were used. 1. Kish tissue regeneration co. 2. Knowledge Based Company of Persian Textile Products. Theproliferation rate in MATRIX and control groups were evaluated 12, 24, 84 hours and 7 days after cultureusing MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide ) test. The morphologicevaluation was made after 24 hours incubation with Ethanol and Dehydrated Hexamethyldisilazane(HMDS) using Platinium coated drops. The surface and circularity of cells was examined byelectronic microscope. Results: The results for DAPI and MTT index was similar in 3 groups(P<0. 4). After 1 week thecontrol group rate was 2. 66 ± 0. 001, fist group, 2. 09 ± 0. 001 and second group, 1. 64± 0. 17. Theresult was statistically signi􀏐 icant. (P<0. 1)Conclusion: It seems, in experimental status, both MATRIX was similar in endothelia celladhesion. But, proliferation rate of ENDOTHELIAL CELLs was more in MATRIX 1 v. s MATRIX 2 after 7days.

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APA: Copy

MAGHADAS, OMID, JAFARI, EHSAN, BAKHSHI, BORHAN, & HARANDI, MOHAMAD. (2019). COMPARISON OF TWO COMMERCIAL DERMAL MATRIX ON BIOLOGICAL BEHAVIOR OF ENDOTHELIAL CELLS(IN VITRO). JOURNAL OF RESEARCH IN DENTAL SCIENCES, 15(4 (58) ), 218-226. SID. https://sid.ir/paper/184669/en

Vancouver: Copy

MAGHADAS OMID, JAFARI EHSAN, BAKHSHI BORHAN, HARANDI MOHAMAD. COMPARISON OF TWO COMMERCIAL DERMAL MATRIX ON BIOLOGICAL BEHAVIOR OF ENDOTHELIAL CELLS(IN VITRO). JOURNAL OF RESEARCH IN DENTAL SCIENCES[Internet]. 2019;15(4 (58) ):218-226. Available from: https://sid.ir/paper/184669/en

IEEE: Copy

OMID MAGHADAS, EHSAN JAFARI, BORHAN BAKHSHI, and MOHAMAD HARANDI, “COMPARISON OF TWO COMMERCIAL DERMAL MATRIX ON BIOLOGICAL BEHAVIOR OF ENDOTHELIAL CELLS(IN VITRO),” JOURNAL OF RESEARCH IN DENTAL SCIENCES, vol. 15, no. 4 (58) , pp. 218–226, 2019, [Online]. Available: https://sid.ir/paper/184669/en

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