EVALUATION OF DISK DIFFUSION AND BROTH MICRODILUTION METHODS FOR FLUCONAZOLE SUSCEPTIBILITY TESTING IN ONE GROUP OF CANDIDA SPP. IN TEHRAN

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NEMATI SHIRZI L.; SHAMS GHAHFAROKHI M.; YADGARI M.H.

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Journal: DANESHVAR MEDICINE Year:2008 | Volume:15 | Issue:73 Page(s): 51-58

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Information Journal Paper

Title

EVALUATION OF DISK DIFFUSION AND BROTH MICRODILUTION METHODS FOR FLUCONAZOLE SUSCEPTIBILITY TESTING IN ONE GROUP OF CANDIDA SPP. IN TEHRAN

Author(s)

NEMATI SHIRZI L. | SHAMS GHAHFAROKHI M. | YADGARI M.H. | Issue Writer Certificate

Keywords

CANDIDAQ2

FLUCONAZOLEQ3

DISK DIFFUSIONQ2

BROTH MICRO DILUTIONQ3

Abstract

Introduction: CANDIDA species have various degrees of susceptibility to antifungal drugs. Thus, detection of antifungal treatment by susceptibility testing methods as a routine test is quit crucial.Materials & Method: A comparison of the DISK DIFFUSION method (D.D) as screening test and the reference broth microdilution method (B.M.D) for FLUCONAZOLE was performed according to national committee for clinical laboratory standards (NCCLS) with106 in one group of CANDIDA spp. in Tehran. Result: In D.D and B.M.D 69.81% and 61.32% of isolates were susceptible(S), whereas 22.6% and 24.52% showed dose-dependent susceptibility (S-DD), and 15.09% and 14.15% were resistant (R), respectively. Susceptibility ranking to FLUCONAZOLE obtained with all yeasts tested was C.dubliniensis » C.parapsilosis » C.lusittani » C.kefyr > C.albicans > C.tropicalis > C.glabera > C.krusei. Conclusion: C.krusei and C.glaberata showed the highest rate of S-DD/R among all species tested. A total correlation between two methods was measured as 94.34% (p<0.05). The D.D method is recommended as a rapid, simple and reliable screening test for detection of susceptibility and compatible with the gold standard method (B.M.D).

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APA: Copy

NEMATI SHIRZI, L., SHAMS GHAHFAROKHI, M., & YADGARI, M.H.. (2008). EVALUATION OF DISK DIFFUSION AND BROTH MICRODILUTION METHODS FOR FLUCONAZOLE SUSCEPTIBILITY TESTING IN ONE GROUP OF CANDIDA SPP. IN TEHRAN. DANESHVAR MEDICINE, 15(73), 51-58. SID. https://sid.ir/paper/30827/en

Vancouver: Copy

NEMATI SHIRZI L., SHAMS GHAHFAROKHI M., YADGARI M.H.. EVALUATION OF DISK DIFFUSION AND BROTH MICRODILUTION METHODS FOR FLUCONAZOLE SUSCEPTIBILITY TESTING IN ONE GROUP OF CANDIDA SPP. IN TEHRAN. DANESHVAR MEDICINE[Internet]. 2008;15(73):51-58. Available from: https://sid.ir/paper/30827/en

IEEE: Copy

L. NEMATI SHIRZI, M. SHAMS GHAHFAROKHI, and M.H. YADGARI, “EVALUATION OF DISK DIFFUSION AND BROTH MICRODILUTION METHODS FOR FLUCONAZOLE SUSCEPTIBILITY TESTING IN ONE GROUP OF CANDIDA SPP. IN TEHRAN,” DANESHVAR MEDICINE, vol. 15, no. 73, pp. 51–58, 2008, [Online]. Available: https://sid.ir/paper/30827/en

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