Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Siasi Torbati elham; Asaei Yeganeh; NOWROOZI JAMILEH

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES Year:2020 | Volume:8 | Issue:2 Page(s): 1-13

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

665

Download:

Cites:

Information Journal Paper

Title

Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples

Author(s)

Siasi Torbati elham | Asaei Yeganeh | NOWROOZI JAMILEH | Issue Writer Certificate

Keywords

Pseudomonas aeruginosaQ2

TEM geneQ1

ImipenemQ1

Real-time PCRQ1

Abstract

Background & Aim: Pseudomonas aeruginosa strains that were resistance to majority of commonly used antibiotics were caused problem in treatment of these infections. Imipenem is the excessive potential antibiotic for elimination of antibiotic resistance isolates of these bacteria. Aim of this study was, identification of Imipenem effect on TEM beta-lactamase gene expression in resistant to antibiotic, Pseudomonas aeruginosa strains, from burn wound samples, by Real-time PCR. Methods: In descriptive cross-sectional study, 100 burn wound samples were collected from Tehran hospitals. Pseudomonas aeruginosa isolates, were found from samples. After DNA extraction by PCR reaction, were isolated TEM gene from samples contained this bacteria, and were defined Imipenem MIC measure, for them. Then, were compared, TEM beta-lactamase gene expression, among Imipenem-treated and untreated Pseudomonas aeruginosa strains, by Real-time PCR Results: From 100 wound samples, 60 isolates (60%) were infected by Pseudomonas aeruginosa and in 34 samples (56. 6%) of Pseudomonas aeruginosa isolated, were presence TEM beta-lactamase gene. Expression of TEM gene, 78. 68% decreased in Imipenem-treated Pseudomonas aeruginosa strains, compare by untreated strains. Conclusion: According of this study finding, from Imipenem by anti-beta-lactamase action that was interested on reduction of TEM gene expression, could be used in control of antibiotic resistance Pseudomonas aeruginosa strains.

Cites

No record.

References

Cite

APA: Copy

Siasi Torbati, elham, Asaei, Yeganeh, & NOWROOZI, JAMILEH. (2020). Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples. JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES, 8(2 ), 1-13. SID. https://sid.ir/paper/370975/en

Vancouver: Copy

Siasi Torbati elham, Asaei Yeganeh, NOWROOZI JAMILEH. Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples. JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;8(2 ):1-13. Available from: https://sid.ir/paper/370975/en

IEEE: Copy

elham Siasi Torbati, Yeganeh Asaei, and JAMILEH NOWROOZI, “Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples,” JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES, vol. 8, no. 2 , pp. 1–13, 2020, [Online]. Available: https://sid.ir/paper/370975/en

Related Journal Papers