INVESTIGATION THE EFFECT OF MICELLE NANOPARTICLES CONTAINING CURCUMIN ON CIPROFLOXACIN RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA AND ON MEXC AND MEXD GENES EXPRESSION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

IMANI PIRSARAEI BOZORGMEHR; RANJI NAJMEH; ASADPOUR LEILA

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2018 | Volume:21 | Issue:2 (131) Page(s): 10-20

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

953

Download:

Cites:

Information Journal Paper

Title

INVESTIGATION THE EFFECT OF MICELLE NANOPARTICLES CONTAINING CURCUMIN ON CIPROFLOXACIN RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA AND ON MEXC AND MEXD GENES EXPRESSION

Author(s)

IMANI PIRSARAEI BOZORGMEHR | RANJI NAJMEH | ASADPOUR LEILA | Issue Writer Certificate

Keywords

CIPROFLOXACINQ2

CURCUMINQ1

MEXCQ1

MEXDQ1

MICELLE NANOPARTICLESQ1

PSEUDOMONAS AERUGINOSAQ1

Abstract

Background: PSEUDOMONAS AERUGINOSA is an opportunistic gram-negative bacterium that is a major cause of nosocomial infections such as severe burns. CURCUMIN is the main component of turmeric (Curcuma longa) that has anti-cancer and anti-inflammatory effects. The aim of this study was to evaluate antibacterial effect of CURCUMIN in CIPROFLOXACIN resistant isolates of PSEUDOMONAS AERUGINOSA through MEXC and MEXD gene expression.Materials and Methods: In this descriptive-analytical study, PSEUDOMONAS AERUGINOSA strains were obtained from hospitals and laboratories in Guilan province. After disc difusion and MIC tests, four CIPROFLOXACIN resistant strains of PSEUDOMONAS AERUGINOSA were treated by CIPROFLOXACIN (1.2MIC) only (control sample) and in the combination with CURCUMIN encapsulated in MICELLE NANOPARTICLES (test sample). After 24h, RNA extraction and cDNA synthesis was performed. Then, the expression of MEXC and MEXD genes was evaluated quantitatively by Real-time PCR method in CURCUMIN treated and untreated cells.Results: This study showed that combination of CIPROFLOXACIN (1.2 MIC) with CURCUMIN encapsulated in MICELLE NANOPARTICLES led to approximately 50% of growth inhibition in PSEUDOMONAS AERUGINOSA. In treated cells with CURCUMIN and CIPROFLOXACIN compared to treated cells with CIPROFLOXACIN alone, MEXC and MEXD genes were significantly (p<0.05) downregulated>0.65 fold in three isolates and>0.1 fold in four isolates, respectively.Conclusion: Our results suggested that CURCUMIN encapsulated in MICELLE NANOPARTICLES combined with 1.2 MIC value of CIPROFLOXACIN inhibits the growth of PSEUDOMONAS AERUGINOSA through reducing MEXC and MEXD expression.

Cites

Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples

References

Cite

APA: Copy

IMANI PIRSARAEI, BOZORGMEHR, RANJI, NAJMEH, & ASADPOUR, LEILA. (2018). INVESTIGATION THE EFFECT OF MICELLE NANOPARTICLES CONTAINING CURCUMIN ON CIPROFLOXACIN RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA AND ON MEXC AND MEXD GENES EXPRESSION. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 21(2 (131) ), 10-20. SID. https://sid.ir/paper/69153/en

Vancouver: Copy

IMANI PIRSARAEI BOZORGMEHR, RANJI NAJMEH, ASADPOUR LEILA. INVESTIGATION THE EFFECT OF MICELLE NANOPARTICLES CONTAINING CURCUMIN ON CIPROFLOXACIN RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA AND ON MEXC AND MEXD GENES EXPRESSION. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2018;21(2 (131) ):10-20. Available from: https://sid.ir/paper/69153/en

IEEE: Copy

BOZORGMEHR IMANI PIRSARAEI, NAJMEH RANJI, and LEILA ASADPOUR, “INVESTIGATION THE EFFECT OF MICELLE NANOPARTICLES CONTAINING CURCUMIN ON CIPROFLOXACIN RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA AND ON MEXC AND MEXD GENES EXPRESSION,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 21, no. 2 (131) , pp. 10–20, 2018, [Online]. Available: https://sid.ir/paper/69153/en

Related Journal Papers