FLOWCYTOMETRIC ASSESSMENT OF MONOCYTE-DERIVED DENDRITIC CELLEFFICIENCY FOR TUMOR CELL LYSATE UPTAKE IN CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS

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KOUKHAEI P.; MAHDIAN R.; PAK F.; SHAHBAZI SH.; SEDIGHI MOGHADAM B.

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Journal: Koomesh Year:2003 | Volume:4 | Issue:1-2 Page(s): 29-38

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Title

FLOWCYTOMETRIC ASSESSMENT OF MONOCYTE-DERIVED DENDRITIC CELLEFFICIENCY FOR TUMOR CELL LYSATE UPTAKE IN CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS

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Keywords

FLOWCYTOMETRYQ4

DENDRITIC CELLQ4

CHRONIC LYMPHOCYTIC LEUKEMIAQ3

LYSATEQ4

TUMOR CELL LYSATEQ4

Abstract

Introduction: Dendrite cells (DCs) are the most potent antigen presenting cells in the immune system. They are strongly involved in initiation of primary immune response, autoimmune disease, graft rejection, HIV infection and anti tumor response. However the capability of DCs to activate tumor specific T cells in chronic lymphocyte leukemia (CLL) has been reported, direct demonstration of soluble antigens of tumor LYSATE uptake had not been shown. In this study we have shown uptake of antigen molecules by immature DCs using Flowcytometric techniques.Material and Methods: DCs of two CLL patients and two healthy donors have been evaluated in this study. Peripheral blood monocytes have been purified using mouse anti-CD14coated magnetic beads and then undergone culture process in the presence of rhGM-CSF andrhIL-4. Generated DCs have been assessed for their ability of uptake FITC-labeled proteins of tumoural B cells LYSATE by flow cytometry. Results: Fluorescence intensity in DCs pulsed with FITC-labeled tumor LYSATE, after using surface fluorescence quencher (try pan blue), were clearly more than unpulsed controls. This was true for both CLL patients DCs and healthy donors DCs.Conclusion: Immature DCs are capable to uptake soluble protein molecules of tumor LYSATE prepared from B cells of CLL patients.

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APA: Copy

KOUKHAEI, P., MAHDIAN, R., PAK, F., SHAHBAZI, SH., & SEDIGHI MOGHADAM, B.. (2003). FLOWCYTOMETRIC ASSESSMENT OF MONOCYTE-DERIVED DENDRITIC CELLEFFICIENCY FOR TUMOR CELL LYSATE UPTAKE IN CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS. KOOMESH, 4(1-2), 29-38. SID. https://sid.ir/paper/392011/en

Vancouver: Copy

KOUKHAEI P., MAHDIAN R., PAK F., SHAHBAZI SH., SEDIGHI MOGHADAM B.. FLOWCYTOMETRIC ASSESSMENT OF MONOCYTE-DERIVED DENDRITIC CELLEFFICIENCY FOR TUMOR CELL LYSATE UPTAKE IN CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS. KOOMESH[Internet]. 2003;4(1-2):29-38. Available from: https://sid.ir/paper/392011/en

IEEE: Copy

P. KOUKHAEI, R. MAHDIAN, F. PAK, SH. SHAHBAZI, and B. SEDIGHI MOGHADAM, “FLOWCYTOMETRIC ASSESSMENT OF MONOCYTE-DERIVED DENDRITIC CELLEFFICIENCY FOR TUMOR CELL LYSATE UPTAKE IN CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS,” KOOMESH, vol. 4, no. 1-2, pp. 29–38, 2003, [Online]. Available: https://sid.ir/paper/392011/en

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