Isolation and identification of cpe-positive Clostridium perfringens in bulk and packed dehydrated vegetables

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Ghorchian Sedigheh; DOURAGHI MASOUMEH; RAHIMIFOROUSHANI ABBAS; SOLTAN DALLAL MOHAMMAD MEHDI

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Razi Journal of Medical Sciences Year:2019 | Volume:26 | Issue:8 Page(s): 23-30

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,012

Download:

Cites:

Information Journal Paper

Title

Isolation and identification of cpe-positive Clostridium perfringens in bulk and packed dehydrated vegetables

Author(s)

Ghorchian Sedigheh | DOURAGHI MASOUMEH | RAHIMIFOROUSHANI ABBAS | SOLTAN DALLAL MOHAMMAD MEHDI | Issue Writer Certificate

Keywords

Clostridium perfringensQ1

cpe geneQ2

EnterotoxinQ1

Food poisoningQ2

PCRQ2

Abstract

Background: The Enterotoxin (cpe) gene in C. perfringensis is responsible for Food poisoning and gastroenteritis. The dried vegetables are suitable environment for spore forming bacteria such C. perfringensis, because of spore resistance against harsh environments such as temperature, dehydrate etc. The aim of this study was to isolate and identify cpe-positive C. perfringens in dried vegetable. Methods: In a descriptive study, 140 samples including 70 bulk and 70 packed dried vegetables collected from various part of Tehran were tested. At first samples were inoculated in thioglycolate as enrichment medium, and then cultured into sulfite polymyxin sulfadiazine (SPS). The colonies were identified by phenotypic and biochemical tests and Duplex PCR was carried out for detection of alpha toxin (cpa) and Enterotoxin (cpe) genes. Results: Out of 140 tested samples 13 samples (9. 3%) were identified as C. perfringens in which all of them were positive for cpa but negative for cpe gene. The rate of contamination of packed vegetables was 12. 8% whereas 5. 7% for bulk. Conclusion: The result of this study showed that C. perfringens contaminations in dried packed vegetables is possible, and that might happen during drying and packaging possess.

Cites

No record.

References

Cite

APA: Copy

Ghorchian, Sedigheh, DOURAGHI, MASOUMEH, RAHIMIFOROUSHANI, ABBAS, & SOLTAN DALLAL, MOHAMMAD MEHDI. (2019). Isolation and identification of cpe-positive Clostridium perfringens in bulk and packed dehydrated vegetables. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 26(8 ), 23-30. SID. https://sid.ir/paper/395489/en

Vancouver: Copy

Ghorchian Sedigheh, DOURAGHI MASOUMEH, RAHIMIFOROUSHANI ABBAS, SOLTAN DALLAL MOHAMMAD MEHDI. Isolation and identification of cpe-positive Clostridium perfringens in bulk and packed dehydrated vegetables. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2019;26(8 ):23-30. Available from: https://sid.ir/paper/395489/en

IEEE: Copy

Sedigheh Ghorchian, MASOUMEH DOURAGHI, ABBAS RAHIMIFOROUSHANI, and MOHAMMAD MEHDI SOLTAN DALLAL, “Isolation and identification of cpe-positive Clostridium perfringens in bulk and packed dehydrated vegetables,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 26, no. 8 , pp. 23–30, 2019, [Online]. Available: https://sid.ir/paper/395489/en

Related Journal Papers