ISOLATION OF ESCHERICHIA COLI O157: H7 IN SHEEP MEATS USING CULTURAL AND PCR METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAFAREYAN SEDIGH M.R.; RAHIMI E.; DOOSTI A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahrekord University of Medical Sciences Year:2011 | Volume:13 | Issue:2 Page(s): 61-69

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,217

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF ESCHERICHIA COLI O157: H7 IN SHEEP MEATS USING CULTURAL AND PCR METHOD

Author(s)

JAFAREYAN SEDIGH M.R. | RAHIMI E. | DOOSTI A. | Issue Writer Certificate

Keywords

ESCHERICHIA COLI O157: H7Q2

SHEEP MEATQ2

PCRQ2

Abstract

Background and aim: Escherichia coli O157: H7 is now recognized as an important cause of diarrhea, hemorrhagia colitis and hemolytic-uremic syndrome worldwide. Meat contaminated with animal feces is probably the major source of the E. coli O157: H7. In this study, we investigated the prevalence of E. coli O157: H7 in meat samples of sheep in Isfahan from August 2008 to January 2010.Methods: A total of 148 sheep carcasses in Isfahan slaughterhouse were assessed for E. coli O157: H7 using the standard cultural and polymerase chain reaction (PCR) methods. Bacteriological examinations were performed by using Triptone Soya Broth containing Novobiocin (TSB-n) as an enrichment media and then sorbitol MacConkey agar plates supplemented with Cefixime and Tellurite (CT-SMAC) a selective plating media. Suspected colonies of E. coli O157: H7, identified by bacteriological methods, were tested by PCR.Results: Using cultural method, 43 (29.1%) and 10 (6.8%) samples were positive for E. coli and E. coli O157: H7, respectively.Only 5 sorobitol negative E. coli strains were identified as E. coli O157: H7, using polymerase chain reaction. The seasonal prevalence of E. coli O157: H7 in samples were 0-9.7% and it was at its highest level in Spring and Summer.Conclusion: These results indicate that sheep can be a reservoir for E. coli O157: H7 and SHEEP MEAT may serve as a vehicle for the pathogen transmission to human.

Cites

No record.

References

No record.

Cite

APA: Copy

JAFAREYAN SEDIGH, M.R., RAHIMI, E., & DOOSTI, A.. (2011). ISOLATION OF ESCHERICHIA COLI O₁₅₇: H₇IN SHEEP MEATS USING CULTURAL AND PCR METHOD. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 13(2), 61-69. SID. https://sid.ir/paper/58293/en

Vancouver: Copy

JAFAREYAN SEDIGH M.R., RAHIMI E., DOOSTI A.. ISOLATION OF ESCHERICHIA COLI O₁₅₇: H₇IN SHEEP MEATS USING CULTURAL AND PCR METHOD. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2011;13(2):61-69. Available from: https://sid.ir/paper/58293/en

IEEE: Copy

M.R. JAFAREYAN SEDIGH, E. RAHIMI, and A. DOOSTI, “ISOLATION OF ESCHERICHIA COLI O₁₅₇: H₇IN SHEEP MEATS USING CULTURAL AND PCR METHOD,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 13, no. 2, pp. 61–69, 2011, [Online]. Available: https://sid.ir/paper/58293/en

Related Journal Papers