DETERMINATION OF CTX-M GENES IN ENTEROBACTERIACEAE PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASE USING PCR METHOD

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TORSHIZI R.; ZAMANZAD B.; MOKHTAREYAN K.; KARIMI A.

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Journal: Journal of Shahrekord University of Medical Sciences Year:2011 | Volume:13 | Issue:3 Page(s): 9-17

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Information Journal Paper

Title

DETERMINATION OF CTX-M GENES IN ENTEROBACTERIACEAE PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASE USING PCR METHOD

Author(s)

TORSHIZI R. | ZAMANZAD B. | MOKHTAREYAN K. | KARIMI A. | Issue Writer Certificate

Keywords

CTX-M GENSQ2

ENTEROBACTERIACEAEQ2

EXTENDED-SPECTRUM β-LACTAMASESQ2

POLYMERASE CHAIN REACTIONQ2

Abstract

Background and aims: Extended-spectrum beta-lactamase of CTX-M-type is considered as an important mechanism resistant to cephalosporin in the gram-negative patogene and is wildly growing. ENTEROBACTERIACEAE species are able to produce extended-spectrum beta-lactamases (ESBLs). The aim of this study was to detect the prevalence of CTX-M genes in ESBLs producing enteric bacteria isolated in Shahrekord educational hospitals using POLYMERASE CHAIN REACTION method.Methods: In this analytical-descriptive study, antibacterial susceptibility patterns of 325 gram negative bacteria to Cefotaxim, Ceftazidim, Cefterixon and Azteronam tested using disk diffusion (Kirby-Buer) method. In addition, confirmatory tests for detecting ESBLs phenotypes were performed using Ceftazidim-clavulanic acid and Cefotaxim-clavulanic acid combination disks (MAST). The presence of CTX-M gene was assessed using PCR.Results: Bacteria strains isolated in this study were Escherichia coli (59.4%), Klebsiella pneumoniae (30.4%), Enterobacter spp. (15.4%) and Proteus spp. (2.2%). Confirmatory phenotypic test showed that 28% of the strains were ESBL positive. The prevalence of CTX-M gene in isolated ENTEROBACTERIACEAE was 50.5 %.Conclusion: High frequency of CTX-M gene in ESBL producing isolates indicates that this enzyme plays an important role in resistance to betalactam containing antibiotics.

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APA: Copy

TORSHIZI, R., ZAMANZAD, B., MOKHTAREYAN, K., & KARIMI, A.. (2011). DETERMINATION OF CTX-M GENES IN ENTEROBACTERIACEAE PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASE USING PCR METHOD. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 13(3), 9-17. SID. https://sid.ir/paper/58827/en

Vancouver: Copy

TORSHIZI R., ZAMANZAD B., MOKHTAREYAN K., KARIMI A.. DETERMINATION OF CTX-M GENES IN ENTEROBACTERIACEAE PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASE USING PCR METHOD. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2011;13(3):9-17. Available from: https://sid.ir/paper/58827/en

IEEE: Copy

R. TORSHIZI, B. ZAMANZAD, K. MOKHTAREYAN, and A. KARIMI, “DETERMINATION OF CTX-M GENES IN ENTEROBACTERIACEAE PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASE USING PCR METHOD,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 13, no. 3, pp. 9–17, 2011, [Online]. Available: https://sid.ir/paper/58827/en

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