FRACTIONAL PURIFICATION AND KINETIC PARAMETERS (KM AND VMAX) OF POLYPHENOL OXIDASE EXTRACTED FROM THREE SEGMENTS OF SOLANUM MELONGENAS AND MUSA SAPIETUM FRUITS

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CHIKEZIE PAUL CHIDOKA; AKUWUDIKE ADAEZE ROSE; CHIKEZIE CHINWENDU MAUREEN; IBEGBULEM CHIEDOZIE ONYEJIAKA

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Journal: IRANIAN JOURNAL OF PLANT PHYSIOLOGY Year:2013 | Volume:3 | Issue:3 Page(s): 703-710

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Title

FRACTIONAL PURIFICATION AND KINETIC PARAMETERS (KM AND VMAX) OF POLYPHENOL OXIDASE EXTRACTED FROM THREE SEGMENTS OF SOLANUM MELONGENAS AND MUSA SAPIETUM FRUITS

Author(s)

CHIKEZIE PAUL CHIDOKA | AKUWUDIKE ADAEZE ROSE | CHIKEZIE CHINWENDU MAUREEN | IBEGBULEM CHIEDOZIE ONYEJIAKA | Issue Writer Certificate

Keywords

POLYPHENOL OXIDASE

SOLANUM MELONGENAS

MUSA SAPIETUM

KINETICS PARAMETERS

Abstract

POLYPHENOL OXIDASE (PPO) was extracted from three segments of SOLANUM MELONGENAS and MUSA SAPIETUM fruits and partially purified. The specific activity of PPO was measured at each purification step to ascertain level of enzyme purity. In all cases, PPO conformed to Michaelis-Menten kinetics, showing different values of KINETICS PARAMETERS. Michaelis-Menten constant (PPOKm) of S. melongenas mid-section and anterior segments showed no significant difference (p<0.05), whereas the posterior gave PPOKm=4.6±0.49 mM (p>0.05). Maximum PPO activity (PPOVmax) was highest in the posterior segment: PPOVmax=0.602±0.09 U. Mid-section of M. sapietum exhibited the highest Km value (PPOKm=5.8±0.69 mM) compared with the anterior (PPOKm=3.9±0.69 mM) (p>0.05) and posterior PPOKm=4.9±0.11 mM segments (p<0.05). Overall, M. sapietum PPOKm values were relatively higher than those of S. melongenas. Posterior S. melongenas exhibited the highest PPOVmax=0.602±0.09 U, whereas the lowest value was registered in the anterior segment of M. sapietum PPOVmax=0.234±0.09 U. Substrate specificity for PPO (PPOVmax/Km) extracted from various segments of S. melongenas was in the increasing order of Mid-section>Posterior>Anterior, whereas that of M. sapietum was Mid-section>Anterior>Posterior. PPOVmax/Km between the two fruits showed strong positive correlation (r=0.862339). Catechol was a better substrate for PPOS. melongenas than PPOM. sapietum. The experimentally observed kinetic parameters of S. melongenas and M. sapietum signified the presence of PPO isoenzymes and non-uniform distribution of PPO in the two fruits.

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APA: Copy

CHIKEZIE, PAUL CHIDOKA, AKUWUDIKE, ADAEZE ROSE, CHIKEZIE, CHINWENDU MAUREEN, & IBEGBULEM, CHIEDOZIE ONYEJIAKA. (2013). FRACTIONAL PURIFICATION AND KINETIC PARAMETERS (K_M AND V_MAX) OF POLYPHENOL OXIDASE EXTRACTED FROM THREE SEGMENTS OF SOLANUM MELONGENAS AND MUSA SAPIETUM FRUITS. IRANIAN JOURNAL OF PLANT PHYSIOLOGY, 3(3), 703-710. SID. https://sid.ir/paper/625714/en

Vancouver: Copy

CHIKEZIE PAUL CHIDOKA, AKUWUDIKE ADAEZE ROSE, CHIKEZIE CHINWENDU MAUREEN, IBEGBULEM CHIEDOZIE ONYEJIAKA. FRACTIONAL PURIFICATION AND KINETIC PARAMETERS (K_M AND V_MAX) OF POLYPHENOL OXIDASE EXTRACTED FROM THREE SEGMENTS OF SOLANUM MELONGENAS AND MUSA SAPIETUM FRUITS. IRANIAN JOURNAL OF PLANT PHYSIOLOGY[Internet]. 2013;3(3):703-710. Available from: https://sid.ir/paper/625714/en

IEEE: Copy

PAUL CHIDOKA CHIKEZIE, ADAEZE ROSE AKUWUDIKE, CHINWENDU MAUREEN CHIKEZIE, and CHIEDOZIE ONYEJIAKA IBEGBULEM, “FRACTIONAL PURIFICATION AND KINETIC PARAMETERS (K_M AND V_MAX) OF POLYPHENOL OXIDASE EXTRACTED FROM THREE SEGMENTS OF SOLANUM MELONGENAS AND MUSA SAPIETUM FRUITS,” IRANIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 3, no. 3, pp. 703–710, 2013, [Online]. Available: https://sid.ir/paper/625714/en

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