JOURNAL OF VETERINARY PATHOBIOLOGY
Year:2010 | Volume:1 | Issue:3|Papers Count:8

F. hepatica is one of the main hepato-billary tract’s parasite that have been reported  from hosts such as cows, buffalows, camels, goats, donkeys, rabbits and boars. Because this parasite that exists in and passes though different hosts, is affected by imported factors (sex, age, nourishment and immunity ability) it may be transformed into morphometric, electrophoretic and morphologic varieties. In present research, some infected samples were taken from the livers of cows, sheep and goats in slaughterhouse in Karaj, and were washed in PBS (pH= 7.4) after being transported to laboratories. Then these samples were stained to measure the external extremities attentively. The results showed that  there was difference in small and large ruminants. To compare every parameter in 3 various hosts, we wed the method of various one-side analysis. Also we studied the somatic protein bands of cows, sheep and goats with SDS-PAGE method. The results protein bands related to the 3 species (cows, sheep and goats) had the range from 18 up to 63 kDa and were 7 in number. The results displayed the fact that protein bands in various hosts are a little different from one another. A 63 kDa band was observed in cows that it didn’t exist in sheep and goats.

The purpose of this study is to determine and detect the verminous infection of the cattle respiratory system. During the second half of the year 1387 (Sep.23, 2008 – Mar.14, 2009) samples were taken from a total of 1235 slaughtered cattle in 28 sessions which lead to 251 cases of lungs infected with hydatid cysts and 4 cases of lungs infected with Linguatula serrata out of 418 cases of condemned lungs. Based on the results of this study, hydatid cyst is the most prevalent parasitic infection in the respiratory system of cattle which, in addition to the numerous respiratory damages it causes, affects on animal’s growth in qualitative and quantitative ways. Therefore, it can be concluded that cautious healthcare policies based on livestock pharmacotherapy and antiparasitic plans can improve the conditions.

Bovine brucellosis and leptosrirosis are important cause of bovine abortion around the world. Both disease can be serologically diagnosed, but many factors may cause false positive and negative results. Direct methods based on bacteriological isolation are usually employed, but they are difficult, time consuming and dangerous. polymerase chain reaction (PCR) have been successfully described for the detection of brucella spp. and leptospira spp.In this study routine use of multiplex PCR assay developed for the detection of brucella spp. and leptospira spp. DNA directly from the stomach contents of aborted cow fetuses.Simplicity and the possibility of detection of both bactera in a single tube reaction support the use of the mPCR in the routine diagnosis.

Sarcocytosis is a zoonotic disease with Sarcocystis spp as causative agent which has been reported from most areas of the world. In this survey, 120 samples of minced beef meat were sampled and examined using digestion method described by Dubey. The results showed that 112 out of 120 (93.33%) samples were infected with Sarcocystis protozoa. The present survey was performed in Sanandaj city, Kordestan province and according to results and concerning to high rate of infection, it is necessary to pay more attention to meat hygienic control by local veterinary organization.

The effect of probiotic on the growth of various animals such as aquatic animals is one of the important issues in the recent years. The aim of this investigation was to evaluate the effects of probiotic protexin on rainbow trout. 240 pieces of rainbow trout with body weight of 10 gr provided from a producing center with same environment and nutritional conditions and divided into 4 groups. Nutrition diet consisting of probiotic was given. The control group did get probiotic but in treatment groups probiotic was given in the amount of 0.1%, 0.2%, 0.3% in the nutrition. Statidtical analysis was done in days 0, 15, 30, 45. In each treatment group average weight, FCR and survival rate was calculated and in each of the times mentiond above, average of variable compared in four groups. adding amount of 0.3% of probiotic protexin to diet showed the best result to increase the amount of average weight and FCR of trout.(p<0.05).adding amount of 0.3% of probiotic protexin to diet, shows the best result to increase the amount of average weight and FCR of  trout, therefore statistical result showed no significant differences between the treatment groups and the control group .(p<0.05).

Rinderpest (RP), popularly known as cattle plague, is one of the oldest known acute and fatal viral disease of domestic livestock like cattle. Rinderpest virus (RPV), causative agent of rinderpest disease, was purified and concentration of its protein determined by Lowry assay, and hemagglutinin (H) protein of RPV separated by using Triton x-114 detergent.The purification process with the application of 10% poly acrylamide gel and RF calculation the separated H-protein molecular weight is 69 KDa which is compatible with the concerning H-protein.

This study between April 2009 until December 2009 has been done. Place of study is Miyaneh city and statistical society is 300 head of single – toxic.The sampling method was random, from blood smears and animal's skin of marginal ear veins with gimsa staining has been done.Result of   skin test shows that tick infection have in 49/300 or 16.45 percent, which that 18 head of these included studied horses (14.75%) and 31 head of monkeys (17.41%) have tick infection.So 35 head of single – toxic included hyaloma anatolicum anatolicum infection (11.66%) and 20 head have rhipicephalus bursa infection (6.66%), and finally 6 head have haemaphysalis punctata infection. In some times we can see coinfection in some cases. The maximum of infection is hyaloma anatolicum anatolicum infection and in June (11.66%). So in blood smears stained with gimsa staining from five horses (4.1%) babesia equi funded, and no results of babesia caballi, finally no results of babesia in blood smears of monkeys founded.

Objective: This experiment was conducted to examine the effect of Intermittent application with different levels of Echinacea purpurea (EP) on Immune responses and performance of broiler chickens.Materials & methods: In this trial 144 broilers (Ross strain) were randomly assigned to 3 treatments, each in 4 replicates contained 12 broilers. During the present experiment, the broilers received 0, 0.5% or 1% of dried aerial part powder of EP with 3-days application followed by 11 application free days. At 28 and 31 days blood sample were taken and analyzed for immune response. Average daily feed intake (ADFI), feed conversion ratio (FCR) and average daily gain (ADG) were determined at 14, 28 and 42 days.Results & conclusion: Result of this study showed that used of 1% dried aerial part powder of EP had significant effect on ADG, FCR and immune responses against sheep red blood cell of broiler chickens (p<0.05), and used of 0.5 % dried aerial part powder of EP had no significant effect on this parameters, It was concluded that EP could be use as a growth and immune enhancer in broiler diets.