Introduction: While nurses have access to confidential information of patients' records, it is still unknown that how information security training and awareness of information security policy can influence their perceived certainty and severity of sanctions. The current study aimed at examining the impact of nurses’ perception of information security training and awareness of information security policy on their perception of severity and certainty of information security breach penalties in specialized teaching hospitals. Methods: The current descriptive, correlational study was conducted on all the nurses working at specialized training hospitals of Isfahan, Iran as the study population and accordingly, 181 nurses were selected. The data were collected by four questionnaires as information security training developed by D’ Arcy et al., security policy awareness developed by Sohrabi Safa et al., and perceived certainty and severity of sanctions developed by Cheng et al., scored based on a five-option Likert scale. The data were collected using non-random sampling. To assess the validity of the questionnaires, content, face, and construct validity, and to assess their reliability Cronbach’ s alpha were used. Descriptive statistics, including frequency and percentage were used to analyze data with SPSS version 19; the study hypotheses were analyzed by partial least squares regression with SmartPLS M2. 0. Results: The average for security training, security awareness, perceived certainty, and severity of sanctions were 3. 78, 3. 41, 3. 63, and 3. 18, respectively. Nurses' awareness of security policies had a positive and significant impact on their perceived severity (t = 9. 1, P < 0. 01, β =0. 41) and certainty (t = 7. 3, P < 0. 01, β = 0. 35) of breach penalties. Training programs had also a significant impact on their perceived severity (t = 2. 3, P < 0. 05, β = 0. 37) and certainty (t = 2. 8, P < 0. 01, β = 0. 44) of breach penalties. Conclusions: Security training and nurses' awareness of security policies can significantly predict their perceived certainty and severity of sanctions for security breach.