In this research antifungal effects of 6 medicinal plant essence, salvia verticilata, Melissa officinalis, Deracocephalum kotschyi, Peroveskia abrotanoides, Zizophora clinopodioides and Origanum vulgar on growth of post harvest disease agents of grape in north khorassan were studied. Samples were collected from cold rooms and grape gardens in 2011 and 2012 according to symptoms of post harvest disease. The main isolated fungi were some species of Alternaria, Penicillium, Aspergillus, Rhizopus and Botrytis genus. Experiment was carried out in completely randomized design with three replications. Essential oils were extracted from All parts of dried medicinal plants and were diluted by Tween 80 to a ratio of one to one. Comparison of means by Duncan's test showed that all the oils at concentrations of 200 and 300 micro-liters per 100 ml of culture medium caused a significant inhibition of the growth of all fungi. Origanum vulgar essential oil caused the most inhibition of post harvest disease fungi growth as the most average growth of fungi happened in Zero concentration and the Lowest growth was observed in concentrations of 200 and 300 micro-liters essential oil of Origanum vulgar per 100 ml of medium. On the other hand Ziziphora had the lowest growth in above concentration Results of Interaction experiment of essential oils against fungi species showed that Origanum vulgar had the most inhibition effect on Aspergillus niger, Botrytis cinerea, Rhizopus stolonifer, Penicillium solitum, Penicillium expansum, Penicillium citrinum and Alternaria alternata; However Melissa officinalis had the most inhibition effect on Botrytis cinerea. Most of the colonies as the most effective essential oil Tasyrbrqtr Marzan pimple fungus Aspergillus niger, Botrytis cinerea, Rhizopus stolonifer, Penicillium solitum, Penicillium expansum, Penicillium citrinum and Alternaria alternata; Vasans Melissa officinalis is the fungus Botrytis cinerea.