Background & Aims: Breast cancer is one of the most common cancers worldwide and accounts for one third of all cancers in women. Vitamin D and its metabolites have a critical role in bone metabolism and any decrease in synthesis and production of this vitamin causes cancers, including breast and ovarian and colon. In this study, we examined the relationship between FOK1 and BSMI restriction enzymes dealing with breast cancer.Materials & Methods: This study was conducted on 180 women (90 female patients and 90 healthy women). DNA was extracted using salting out method. In the next stage the designed primers were connected to complementary sequences using PCR and in the last stage, restriction enzymes specific places on the DNA sequences were cut using RFLP. Finally the data were analyzed using SPSSV23, chi-square and t-Test.Results: There was no significant difference considering the density of the obtained genotype polymorphisms in both patients and controls (P =0.422). There was a significant difference between the genotypes considering age (P=0.19) and slow the disease progression (P=0.65). In both groups, there was significant association between the variables of age, breastfeeding and age at first pregnancy (p<0.05). There also was association between the use of oral contraceptives with occurrences of the disease (p>0.005).Conclusion: In view of the role of vitamin D receptors in cell cycle control cycles and the whole immune system in breast cancer need to consider other gene polymorphisms with breast cancer is suggested.