INTERPRETATION OF IMMUNOHISTOCHEMISTRY RESULTS OF HER2/NEU EXPRESSION IN INVASIVE BREAST CANCER: EVALUATION OF INTEROBSERVER AND INTRAOBSERVER AGREEMENT

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KADIVAR MARYAM; ASKARZADEH P.; SHAYANFAR N.; SADEGHIPOUR ALI REZA; BAHRAMINEZHAD MARYAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Razi Journal of Medical Sciences Year:2009 | Volume:16 | Issue:62 Page(s): 147-155

Download Full-Text

Persian Verion

View:

3,205

Download:

Cites:

Information Journal Paper

Title

INTERPRETATION OF IMMUNOHISTOCHEMISTRY RESULTS OF HER2/NEU EXPRESSION IN INVASIVE BREAST CANCER: EVALUATION OF INTEROBSERVER AND INTRAOBSERVER AGREEMENT

Author(s)

Keywords

HER2/NEUQ3

INTEROBSERVER AGREEMENTQ3

INVASIVE BREAST CANCERQ2

Abstract

Background and Aim: The accurate determination of HER-2 in INVASIVE BREAST CANCER has become a critical issue, particularly in context of the results of Herceptin adjuvant therapy. The aim of this study was to evaluate inter- and intraobserver reproducibility of assessment of HER2/NEU immunostaining in INVASIVE BREAST CANCER.Materials and Methods: This study was cross sectional and the convenience sampling method was applied. IHC for HER2/NEU was performed on 107 archival invasive breast carcinoma tissues. Protein overexpression was scored independently by 4 pathologists using the criteria recommended by DAKO (0 to 3+ reflecting the intensity and pattern of the immunohistochemical signal present in 10% or more of tumor cells.). After a session in order to train for better evaluation and standardization of the scoring criteria, the samples were evaluated for the second time. A minimum of two months elapsed between the two examinations. The discrepancies in scoring results were reviewed by the 4 observers using a multihead microscope to obtain consensus. The inter- and intraobserver agreements were calculated by kappa statistics.Results: Complete agreement was achieved in 33 (31%) and 40 (37%) of 107 cases in the first and second examinations, respectively. Generally, INTEROBSERVER AGREEMENT was moderate (Kappa=0.44 in the first examination and 0.51 in the second one), with intermediate categories (1+, 2+) showing fair agreement and extreme categories (0, 3+) showing moderate agreement. Intraobserver agreement was also moderate (from the minimum kappa=0.55 to the maximum of 0.60). Considering three groups as negative (0/1+), weakly positive (2+) and strongly positive (3+), INTEROBSERVER AGREEMENT improved but still staying in the moderate level (kappa=0.54 and 0.60 in the first and second time, respectively) with score 2+ group showing the least level of agreement. However, with dividing the cases into two groups as negative (0/1+) and positive (2+/3+), INTEROBSERVER AGREEMENT improved to substantial level (kappa=0.68 and 0.75 in the first and second time, respectively).Also, with this consideration, intraobserver agreement improved to substantial level (0.71 to 0.84). By consensus there were 42 cases (39.2%) that were scored 0, 19 cases (17.8%) scored 1+, 27 cases (25.2%) scored 2+, and 11 cases (10.3%) scored 3+. For 8 cases (7.5%) no consensus was achieved; in 6 of them the problem was placing them into 2+ or 3+ category and in 2 cases the problem was placing them into 0 or 1+ category.Conclusion: Our study showed that reproducibility (interobserver agreement) is moderate for evaluation of HER2 status in invasive breast carcinoma by IHC using DAKO criteria. However, for discrimination between negative and positive state, reproducibility (that is in substantial level) is better.

Cites

No record.

References

No record.

Cite

APA: Copy

KADIVAR, MARYAM, ASKARZADEH, P., SHAYANFAR, N., SADEGHIPOUR, ALI REZA, & BAHRAMINEZHAD, MARYAM. (2009). INTERPRETATION OF IMMUNOHISTOCHEMISTRY RESULTS OF HER2/NEU EXPRESSION IN INVASIVE BREAST CANCER: EVALUATION OF INTEROBSERVER AND INTRAOBSERVER AGREEMENT. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 16(62), 147-155. SID. https://sid.ir/paper/11013/en

Vancouver: Copy

KADIVAR MARYAM, ASKARZADEH P., SHAYANFAR N., SADEGHIPOUR ALI REZA, BAHRAMINEZHAD MARYAM. INTERPRETATION OF IMMUNOHISTOCHEMISTRY RESULTS OF HER2/NEU EXPRESSION IN INVASIVE BREAST CANCER: EVALUATION OF INTEROBSERVER AND INTRAOBSERVER AGREEMENT. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2009;16(62):147-155. Available from: https://sid.ir/paper/11013/en

IEEE: Copy

MARYAM KADIVAR, P. ASKARZADEH, N. SHAYANFAR, ALI REZA SADEGHIPOUR, and MARYAM BAHRAMINEZHAD, “INTERPRETATION OF IMMUNOHISTOCHEMISTRY RESULTS OF HER2/NEU EXPRESSION IN INVASIVE BREAST CANCER: EVALUATION OF INTEROBSERVER AND INTRAOBSERVER AGREEMENT,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 16, no. 62, pp. 147–155, 2009, [Online]. Available: https://sid.ir/paper/11013/en

Related Journal Papers