The main purpose of the present study was to investigate the factor structure, validity and reliability of school organizational health questionnaire (SOHQ) in a sample of Iranian teachers. From among all teachers of Ashkezar and Meybod, 448 teachers were selected by categorical random sampling who completed school organizational health (Hart, et al, 2000), organizational commitment (Mowday, et al, 1979) and School Level Environment questionnaires (Rentoul & Fraser, 1983). The data were analyzed using structural equation modeling, Pearson correlation, and independent t test. The results showed that SOHQ has good face and content validity. The factor structures of 54-, 38-, and 16-item questionnaires of SOHQ were confirmed using confimatory factor analysis.The SOHQ had significant discriminatory validity and also a significant convergent validity with teachersʼ organizational commitment and school organizational climate. Test-retest reliability of 54, 38 and 16- item forms of SOHQ were.71, .78 and.81 respectively. The Cronbach alphas of SOHQ were.94, .87 and.76 in 54, 38 and 16- items forms, respectively. Generally, the results show that the Farsi version of SOHQ has a good validity and reliability in the sample of Iranian teachers participated in the present study.