The aim of this study was to assess toxic heavy metals concentration including Hg, Cd, Ni, Sn, Zn and Fe in canned tunna fish in iran, winter 2009. The sampling was carried out from three different factories in Shushtar, Chabahar and Esfahan. The heavy metals was extracted from the canned tuna using wet digestion method and concentration of the heavy metals were measured by Atomic Absorption Spectrophotometer Perkin Elmer 4100. Data were analyzed with SPSS17 software in terms of t-test which determined existence or non-existence of the significant difference in level 5 precent (P=0.05). The highest concentration of Hg, Cd, Ni and Sn 48.73±7.55, 336.23±11.08, 171.66±31.21 and 82.20±11.73 mg/Kg was the lowest concentration of Hg, Cd, Ni and Sn 37.53±1.33, 141.43±5.45, 54.99±4.52 and 28.60±1.85 mg/Kg. The highest concentration of Fe and Zn 7.63±0.04 and 5.36±0.82 mg/Kg was the lowest concentration of Fe and Zn 4.29±0.45 and 2.84±0.42 mg/kg.